Krylov B V, Vilin Y Y, Katina I E, Podzorova S A
IP Pavlov Institute of Physiology, Russian Academy of Sciences, St Petersburg.
Neurosci Behav Physiol. 2000 May-Jun;30(3):331-7. doi: 10.1007/BF02471787.
The effects of ethanol on tetrodotoxin-sensitive (TTXs) and tetrodotoxin-resistant (TTXr) sodium channels in rat spinal ganglia were studied using a patch-clamp method. Application of ethanol (10 and 100 mM) to both sides of membranes resulted in decreases in the reversion potentials of both types of sodium channels. In the case of TTXr channels, ethanol decreased their selectivity in relation to Na ions and altered the sequence of ion selectivity of these channels for different cations from row X to row XI of the Eisenman selectivity classification. It is suggested that this change in ion selectivity is associated with ethanol-induced disruption of hydrogen bonds which stabilize the spatial structure of ion channel macromolecules, which may lead to changes in the steric parameters of the pores formed by these channels.
采用膜片钳方法研究了乙醇对大鼠脊髓神经节中河豚毒素敏感（TTXs）和河豚毒素耐受（TTXr）钠通道的影响。在膜两侧施加乙醇（10和100 mM）导致两种类型钠通道的反转电位降低。对于TTXr通道，乙醇降低了它们对Na离子的选择性，并改变了这些通道对艾森曼选择性分类中从X行到XI行不同阳离子的离子选择性顺序。有人认为，这种离子选择性的变化与乙醇诱导的氢键破坏有关，氢键稳定了离子通道大分子的空间结构，这可能导致这些通道形成的孔的空间参数发生变化。
