Sharpe C R, Goldstone K
Department of Zoology, University of Cambridge, UK.
Development. 1997 Jan;124(2):515-23. doi: 10.1242/dev.124.2.515.
Retinoid receptors, which are members of the nuclear hormone receptor superfamily, act as ligand-dependent transcription factors. They mediate the effects of retinoic acid primarily as heterodimers of retinoic acid receptors (RARs) and retinoid X receptors (RXRs). To analyse their function, xRXR beta synthetic mRNA was injected into Xenopus embryos in combination with normal and mutated xRAR alpha transcripts. Two informative phenotypes are reported here. Firstly, over-expression of xRXR beta with xRAR alpha results in the formation of ectopic primary neurons. Secondly, blocking retinoid signalling with a mutated xRAR alpha results in a lack of primary neurons. These two phenotypes, from contra-acting manipulations, indicate a role for retinoid signalling during neurogenesis.
类视黄醇受体是核激素受体超家族的成员，作为配体依赖性转录因子发挥作用。它们主要作为视黄酸受体（RARs）和类视黄醇X受体（RXRs）的异二聚体介导视黄酸的作用。为了分析它们的功能，将xRXRβ合成mRNA与正常和突变的xRARα转录本一起注射到非洲爪蟾胚胎中。本文报道了两种有意义的表型。首先，xRXRβ与xRARα的过度表达导致异位初级神经元的形成。其次，用突变的xRARα阻断类视黄醇信号传导导致初级神经元缺乏。这两种来自相反操作的表型表明类视黄醇信号在神经发生过程中起作用。
