Ohtani K, Bando M, Swe T, Banu S, Oe M, Hayashi H, Shimizu T
Department of Microbiology, Institute of Basic Medical Sciences, University of Tsukuba, Japan.
FEMS Microbiol Lett. 1997 Jan 1;146(1):155-9. doi: 10.1111/j.1574-6968.1997.tb10186.x.
The 3'-flanking region of the beta-galactosidase gene (pbg), which is located downstream of the perfringolysin O gene (pfoA), and the 5'-flanking region of the collagenase gene (colA) of Clostridium perfringens strains NCTC8237 and 13, respectively, were analyzed. Southern analysis revealed that the colA gene is located 6.5 kb downstream of the pbg gene in the chromosome of C. perfringens. Sequence analysis showed that between the pbg and colA genes were the arcABDC and ahrC genes, whose putative products were quite similar to enzymes of the arginine deiminase pathway of Pseudomonas aeruginosa and the arginine repressor/activator of Bacillus subtilis, respectively. It is concluded that the genomic structure of the pfoA-colA region consists of pfoR-pfoA-ORF54-pbg-arcABDC-ahrC-colA.
分别对产气荚膜梭菌菌株NCTC8237和13的β-半乳糖苷酶基因（pbg）的3'侧翼区域（位于穿孔毒素O基因（pfoA）下游）和胶原酶基因（colA）的5'侧翼区域进行了分析。Southern分析显示，在产气荚膜梭菌染色体中，colA基因位于pbg基因下游6.5 kb处。序列分析表明，在pbg和colA基因之间是arcABDC和ahrC基因，其推定产物分别与铜绿假单胞菌精氨酸脱亚胺酶途径的酶和枯草芽孢杆菌的精氨酸阻遏物/激活剂非常相似。得出结论，pfoA - colA区域的基因组结构由pfoR - pfoA - ORF54 - pbg - arcABDC - ahrC - colA组成。
