产气荚膜梭菌中β-半乳糖苷酶基因（pbg）的转录分析。

Transcriptional analysis of the beta-galactosidase gene (pbg) in Clostridium perfringens.

作者信息

Kobayashi T, Shimizu T, Hayashi H

机构信息

Department of Microbiology, University of Tsukuba, Ibaraki, Japan.

出版信息

FEMS Microbiol Lett. 1995 Nov 1;133(1-2):65-9. doi: 10.1111/j.1574-6968.1995.tb07862.x.

DOI:10.1111/j.1574-6968.1995.tb07862.x

PMID:8566714

Abstract

The mode of expression of the beta-galactosidase gene (pbg) of Clostridium perfringens was examined. The pbg gene was transcribed on a single 3.7-kb mRNA. The transcript contained a message for ORF54, located upstream of the pbg gene in the chromosome, indicating that ORF54 and the pbg gene comprise one operon (pbg operon). Expression of the pbg operon was induced by lactose at the transcriptional level. The promoter structure of the pbg operon was characterized by many palindrome structures and direct repeats, which suggests that there might be some catabolite regulation of the expression of the pbg operon in C. perfringens.

摘要

对产气荚膜梭菌β-半乳糖苷酶基因（pbg）的表达模式进行了研究。pbg基因转录在一条单一的3.7 kb mRNA上。该转录本包含位于染色体上pbg基因上游的ORF54的信息，表明ORF54和pbg基因组成一个操纵子（pbg操纵子）。pbg操纵子的表达在转录水平上由乳糖诱导。pbg操纵子的启动子结构具有许多回文结构和直接重复序列，这表明产气荚膜梭菌中pbg操纵子的表达可能存在某种分解代谢物调控。