Kurose K, Sakihama H, Mori O, Sasai Y
Department of Dermatology, Kurume University School of Medicine, Japan.
Acta Histochem. 1996 Jan;98(1):39-46. doi: 10.1016/S0065-1281(96)80048-1.
Quantitative and qualitative changes of nuclear DNA were analyzed by microfluorometry in normal human epidermis during terminal differentiation. Normal human epidermis from five healthy volunteers was separated by an EDTA-trypsin method and fractionated by Percoll density gradient. Staining was carried out with either Feulgen reaction, Acridine Orange or 4'-6-diamidino-2-phenylindole (DAPI). When the cells with lower density (granular layer cells) were stained with Acridine Orange or DAPI, the greatest population was found at lower value of DNA than that in the cells stained with Feulgen reaction. After thermal denaturation procedure, a ratio of double-stranded DNA to single-stranded DNA was highest in the cells with lower density. These results seem to suggest the qualitative and quantitative changes of nuclear DNA during terminal differentiation of human epidermal keratinocytes.
通过显微荧光测定法分析了正常人表皮在终末分化过程中核DNA的定量和定性变化。采用EDTA-胰蛋白酶法分离了五名健康志愿者的正常人表皮，并通过Percoll密度梯度进行分级。使用福尔根反应、吖啶橙或4'-6-二脒基-2-苯基吲哚（DAPI）进行染色。当用吖啶橙或DAPI对低密度细胞（颗粒层细胞）进行染色时，发现DNA值低于用福尔根反应染色的细胞。热变性处理后，低密度细胞中双链DNA与单链DNA的比例最高。这些结果似乎表明人表皮角质形成细胞终末分化过程中核DNA的定性和定量变化。
