Kobayashi Y, Takahashi Y, Chikayama S, Ikeda M, Uoshima N, Tanaka K, Kimura S, Wada K, Sugano T, Ozawa M, Kondo M
1st Department of Internal Medicine, Kyoto Prefectural University of Medicine.
Rinsho Byori. 1998 Feb;46(2):139-43.
We developed a new microfluorometric method to measure the DNA and RNA contents of individual megakaryocytes using acridine orange (AO) staining in human bone marrow smears. Some bone marrow smears were fixed with ethanol and treated with pretreatment solution and then stained with an AO staining solution. The DNA content was assayed by measuring the green fluorescence and the RNA content was assayed by measuring the red fluorescence under B excitation with microfluorometer using peripheral lymphocytes as the control. The ploidy peak was shown to be 16N in all of 5 normal controls, but it was 8N in the patient with refractory anemia with excess of blasts in transformation (RAEB-T). There was not difference in the RNA content/DNA content ratios (RI) between each ploidy in the normal controls. The RI of the patient with RAEB-T was lower than that of the normal controls. The measurement of the DNA-RNA contents may be useful as a new megakaryocytic parameter.
我们开发了一种新的显微荧光测定方法，用于通过吖啶橙（AO）染色测量人骨髓涂片中单个巨核细胞的DNA和RNA含量。一些骨髓涂片用乙醇固定，用预处理溶液处理，然后用AO染色溶液染色。以周边淋巴细胞作为对照，使用显微荧光计在B激发下通过测量绿色荧光来测定DNA含量，通过测量红色荧光来测定RNA含量。在所有5名正常对照中，倍性峰值显示为16N，但在转化型原始细胞过多的难治性贫血（RAEB-T）患者中为8N。正常对照中各倍性之间的RNA含量/DNA含量比值（RI）没有差异。RAEB-T患者的RI低于正常对照。DNA-RNA含量的测量作为一种新的巨核细胞参数可能是有用的。
