利用单克隆抗体CD42a进行流式细胞术血小板计数。

Flow cytometric platelet enumeration utilizing monoclonal antibody CD42a.

作者信息

Tanaka C, Ishii T, Fujimoto K

机构信息

Scientific Division, R&D, TOA Medical Electronics Co., Ltd, Kobe, Japan.

出版信息

Clin Lab Haematol. 1996 Dec;18(4):265-9.

PMID:9054700

Abstract

We investigated a flow cytometric method with monoclonal antibody CD42a as a potential reference method for platelet enumeration by blood count analysers. Using peripheral blood samples from 25 healthy individuals we obtained significant (P < 0.0001) correlations between the results obtained by a FACScan method and similar cell counters (TOA Medical Electronics, Kobe, Japan): r = 0.960 (FACScan vs SSF), r = 0.958 (FACScan vs SE-9000A,B), r = 0.949 (FACScan vs K-4500A) and r = 0.954 (FACScan vs K-4500B). This flow cytometric method for counting platelets using the monoclonal antibody CD42a can be used to check the calibration of the platelet count by blood cell analysers.

摘要

我们研究了一种使用单克隆抗体CD42a的流式细胞术方法，作为血细胞分析仪进行血小板计数的潜在参考方法。我们使用来自25名健康个体的外周血样本，通过FACScan方法获得的结果与类似的血细胞计数器（日本神户的东亚医疗电子公司）之间存在显著相关性（P < 0.0001）：r = 0.960（FACScan与SSF），r = 0.958（FACScan与SE - 9000A、B），r = 0.949（FACScan与K - 4500A）以及r = 0.954（FACScan与K - 4500B）。这种使用单克隆抗体CD42a计数血小板的流式细胞术方法可用于检查血细胞分析仪血小板计数的校准。