Vives Corrons J L, Besson I, Villamor N, Aymerich M, Marín J L, Jou J M
Servei d'Hematologia Biològica, Hospital Clínic i Provincial, Universitat de Barcelona.
Sangre (Barc). 1994 Jun;39(3):197-201.
To compare the results of the monocyte count provided by autoanalysers with those attained with flow cytometry.
Fifty-six blood samples (EDTA-K3) from the emergency laboratory were studied. The automatic percentage of monocytes attained by four autoanalysers, STKS (Coulter-IZASA), Technicon H*2 (Bayer Diagnostics), Cell-Dyn-3000 (Abbott Diagnostics) and NE-8000 (TOA-Sysmex) was confronted with that provided by the flow cytometry analysis performed with a FACScan (Becton-Dickinson) using monoclonal antibodies of myeloid-monocytic lineage (CD14, CD16). The morphologic observation of the blood smear in accordance with the H20-A protocol of the National Committee for Clinical Laboratory Standards (NCCLS) was used as a reference method. Descriptive statistics plus linear regression analysis, along with the Student's t test for paired data were used for the statistical evaluation of the results.
Significant correlation was found between the systems under study and the flow cytometry method: Technicon H2 (y = 1.79 +/- 0.61x; r = 0.91, p < 0.0001), Coulter STKS (y = 0.61 +/- 0.93X; r = 0.89, p < 0.0001), Cell-Dyn 3000 (y = 0.88 + 0.64x; r = 0.78, p < 0.0001) and Sysmex NE-8000 (y = 4.60 +/- 0.33x; r = 0.52, p < 0.0001). When comparing the percentage of monocytes by means of the Student's t test it was found that Coulter STKS provided the closest results with regard to flow cytometry (13.78 +/- 9.27 vs 14.19 +/- 8.88, p = 0.49), while the closest findings with respect to the reference method were given by Technicon H2 (9.29 +/- 4.78 vs 9.71 +/- 4.77, p = 0.30).
From this analysis, it was concluded that flow cytometry could be an alternative method to conventional optic observation in the evaluation of the differential count of leucocytes, including monocytes.
比较自动分析仪提供的单核细胞计数结果与流式细胞术获得的结果。
研究了来自急诊实验室的56份血液样本（EDTA-K3）。用四台自动分析仪STKS（库尔特-伊扎萨）、Technicon H*2（拜耳诊断）、Cell-Dyn-3000（雅培诊断）和NE-8000（东亚Sysmex）获得的单核细胞自动百分比，与使用FACScan（贝克顿-迪金森）、采用髓系-单核系谱系单克隆抗体（CD14、CD16）进行的流式细胞术分析所提供的结果进行对比。按照美国国家临床实验室标准委员会（NCCLS）的H20-A方案对血涂片进行形态学观察，以此作为参考方法。采用描述性统计、线性回归分析以及配对数据的学生t检验对结果进行统计学评估。
在所研究的系统与流式细胞术方法之间发现了显著相关性：Technicon H2（y = 1.79 ± 0.61x；r = 0.91，p < 0.0001）、库尔特STKS（y = 0.61 ± 0.93X；r = 0.89，p < 0.0001）、Cell-Dyn 3000（y = 0.88 + 0.64x；r = 0.78，p < 0.0001）和Sysmex NE-8000（y = 4.60 ± 0.33x；r = 0.52，p < 0.0001）。通过学生t检验比较单核细胞百分比时发现，就流式细胞术而言，库尔特STKS提供的结果最接近（13.78 ± 9.27对14.19 ± 8.88，p = 0.49），而就参考方法而言，Technicon H2给出的结果最接近（9.29 ± 4.78对9.71 ± 4.77，p = 0.30）。
通过该分析得出结论，在评估包括单核细胞在内的白细胞分类计数时，流式细胞术可作为传统光学观察的替代方法。
