Van Heusden M C, Erickson B A, Pennington J E
Department of Biochemistry, University of Arizona, Tucson 85721, USA.
Arch Insect Biochem Physiol. 1997;34(3):301-12. doi: 10.1002/(SICI)1520-6327(1997)34:3<301::AID-ARCH5>3.0.CO;2-X.
High density lipophorin (HDLp) is the major lipid transport vehicle in insect hemolymph. Using an indirect ELISA, levels of HDLp were measured in the yellow fever mosquito, Aedes aegypti. The level of lipophorin, when normalized to the total weight of the insect, was similar in the different developmental stages. Starvation (access to water only) of adult females did not affect the level of HDLp nor its density when compared to sugar-fed females. On the other hand, blood feeding (of normally sugar-fed females) resulted in a three-fold increase of the HDLp level at 40 h after feeding. This increase was accompanied by a slight but significant increase in the density of HDLp at 24 h after feeding. Ingestion of a lipid-free protein meal or a lipid-supplemented protein meal induced changes in HDLp level and density that were comparable to those induced by ingestion of a blood meal. Ingestion of a blood meal, following starvation (access to water only) from the moment of adult emergence, did not induce an increase in HDLp level. The results presented indicate that, in contrast to other insect species, A. aegypti responds to an increased need for lipid transport in the hemolymph by increasing the amount of HDLp. Arch. Insect Biochem.
高密度脂蛋白（HDLp）是昆虫血淋巴中的主要脂质运输载体。利用间接酶联免疫吸附测定法（ELISA），对黄热病蚊子埃及伊蚊的HDLp水平进行了测量。当根据昆虫的总重量进行标准化时，脂蛋白水平在不同发育阶段相似。与喂食糖的成年雌性相比，成年雌性饥饿（仅提供水）并不影响HDLp的水平及其密度。另一方面，（对正常喂食糖的雌性）进行血液喂食后，在喂食后40小时HDLp水平增加了三倍。这种增加伴随着喂食后24小时HDLp密度的轻微但显著增加。摄入无脂蛋白质餐或补充脂质的蛋白质餐会引起HDLp水平和密度的变化，这些变化与摄入血餐所引起的变化相当。从成年出现时刻起就开始饥饿（仅提供水）后再摄入血餐，并不会导致HDLp水平增加。所呈现的结果表明，与其他昆虫物种不同，埃及伊蚊通过增加HDLp的量来应对血淋巴中对脂质运输增加的需求。《昆虫生物化学杂志》
