Wende G, Fry S C
ICMB, DBS, University of Edinburgh, U.K.
Phytochemistry. 1997 Mar;44(6):1011-8. doi: 10.1016/s0031-9422(96)00648-6.
Partial acid hydrolysis of cell wall material from Festuca arundinacea cell cultures yielded a novel O-feruloylated trisaccharide (3). Treatment of 3 with Driselase, which contains beta- but not alpha-D-xylosidase, released xylose plus the known compound, beta-D-xylopyranosyl-(1-->2)-(5-O-feruloyl)-L-arabinose. Since 3 contained one NaIO4-resistant xylose residue, it was concluded to be beta-D-xylopyranosyl-(1-->3)-beta-D-xylopyranosyl-(1--> 2)-(5-O-feruloyl)-L-arabinose. Partial acid hydrolysis of Festuca cell walls also yielded several higher-M(r) feruloylated oligosaccharides, including a feruloylated pentasaccharide, 4 (sugar composition: Ara + Xyl2 + two non-pentose residues) and a feruloylated heptasaccharide, 5 (Ara + Xyl3 + three non-pentose residues). Compounds 4 and 5 were endogenously O-acetylated but 3 was not. Similar or identical compounds were found in hydrolysates of 20 additional species of the Gramineae. These products represent a series of complex side-chains which, in vivo, are attached via Araf residues to the parent xylan. Their possible biological roles are discussed.
对高羊茅细胞培养物的细胞壁材料进行部分酸水解，得到了一种新型的O-阿魏酰化三糖（3）。用含有β-木糖苷酶但不含α-D-木糖苷酶的里斯酶处理3，释放出木糖和已知化合物β-D-吡喃木糖基-(1→2)-(5-O-阿魏酰基)-L-阿拉伯糖。由于3含有一个对高碘酸钠有抗性的木糖残基，因此得出其为β-D-吡喃木糖基-(1→3)-β-D-吡喃木糖基-(1→2)-(5-O-阿魏酰基)-L-阿拉伯糖。高羊茅细胞壁的部分酸水解还产生了几种更高分子量的阿魏酰化寡糖，包括一种阿魏酰化五糖4（糖组成：Ara + Xyl2 + 两个非戊糖残基）和一种阿魏酰化七糖5（Ara + Xyl3 + 三个非戊糖残基）。化合物4和5内源性地被O-乙酰化，但3没有。在另外20种禾本科植物的水解产物中发现了相似或相同的化合物。这些产物代表了一系列复杂的侧链，在体内，它们通过阿拉伯糖残基连接到母体木聚糖上。讨论了它们可能的生物学作用。
