Viëtor R J, Hoffmann R A, Angelino S A, Voragen A G, Kamerling J P, Vliegenthart J F
Department of Food Science, Agricultural University, Wageningen, Netherlands.
Carbohydr Res. 1994 Feb 17;254:245-55. doi: 10.1016/0008-6215(94)84257-4.
Arabinoxylans consisting of more than 90% of arabinose and xylose were extracted from water-insoluble cell wall material of dehusked barley grain, and degraded with a purified endo-(1-->4)-beta-D-xylanase from Aspergillus awamori. Twelve of the oligosaccharide fragments released were isolated by a combination of size-exclusion and anion-exchange chromatography, and their structures were determined by 1H NMR spectroscopy. The oligosaccharides identified consisted of (1-->4)-linked beta-D-xylopyranosyl residues, some of which were substituted at O-2, at O-3, or at both O-2 and O-3, with alpha-L-arabinofuranosyl groups. Two new structures, a tetrasaccharide and a pentasaccharide, both containing terminal 2-O-arabinofuranosylxylopyranose as a structural element are described.
从去皮大麦籽粒的水不溶性细胞壁材料中提取出阿拉伯糖和木糖含量超过90%的阿拉伯木聚糖，并用来自泡盛曲霉的纯化内切-(1→4)-β-D-木聚糖酶进行降解。通过尺寸排阻色谱和阴离子交换色谱相结合的方法分离出释放的12种寡糖片段，并用1H NMR光谱法确定其结构。鉴定出的寡糖由(1→4)-连接的β-D-木吡喃糖基残基组成，其中一些在O-2、O-3或O-2和O-3处被α-L-阿拉伯呋喃糖基取代。描述了两种新结构，一种四糖和一种五糖，它们都含有末端2-O-阿拉伯呋喃糖基木吡喃糖作为结构元件。
