在克拉维酸存在的情况下生长的肺炎链球菌的异常生理特性及细胞壁组成改变。
Abnormal physiological properties and altered cell wall composition in Streptococcus pneumoniae grown in the presence of clavulanic acid.
作者信息
Severin A, Severina E, Tomasz A
机构信息
Rockefeller University, New York, New York 10021, USA.
出版信息
Antimicrob Agents Chemother. 1997 Mar;41(3):504-10. doi: 10.1128/AAC.41.3.504.
Abstract
Subinhibitory concentrations of clavulanate caused premature induction of stationary-phase autolysis, sensitization to lysozyme, and reductions in the MICs of deoxycholate and penicillin for Streptococcus pneumoniae. In the range of clavulanate concentrations producing these effects, this beta-lactam compound was selectively bound to PBP 3. Cell walls isolated from pneumococci grown in the presence of clavulanate showed increased sensitivity to the hydrolytic action of purified pneumococcal autolysin in vitro. High-performance liquid chromatography analysis of the peptidoglycan isolated from the clavulanate-grown cells showed major qualitative and quantitative changes in stem peptide composition, the most striking feature of which was the accumulation of peptide species carrying intact D-alanyl-D-alanine residues at the carboxy termini. The altered biological and biochemical properties of the clavulanate-grown pneumococci appear to be the consequences of suppressed D,D-carboxypeptidase activity.
摘要
克拉维酸的亚抑制浓度可导致肺炎链球菌过早诱导稳定期自溶、对溶菌酶敏感以及脱氧胆酸盐和青霉素的最低抑菌浓度降低。在产生这些效应的克拉维酸浓度范围内，这种β-内酰胺化合物选择性地与青霉素结合蛋白3结合。从在克拉维酸存在下生长的肺炎球菌中分离的细胞壁在体外对纯化的肺炎球菌自溶素的水解作用表现出更高的敏感性。对从在克拉维酸中生长的细胞中分离的肽聚糖进行高效液相色谱分析表明，茎肽组成发生了主要的定性和定量变化，其中最显著的特征是在羧基末端携带完整D-丙氨酰-D-丙氨酸残基的肽种类的积累。在克拉维酸中生长的肺炎球菌的生物学和生化特性改变似乎是D，D-羧肽酶活性受到抑制的结果。
相似文献
1
Abnormal physiological properties and altered cell wall composition in Streptococcus pneumoniae grown in the presence of clavulanic acid.在克拉维酸存在的情况下生长的肺炎链球菌的异常生理特性及细胞壁组成改变。
Antimicrob Agents Chemother. 1997 Mar;41(3):504-10. doi: 10.1128/AAC.41.3.504.
2
Drastic changes in the peptidoglycan composition of penicillin resistant laboratory mutants of Streptococcus pneumoniae.肺炎链球菌青霉素耐药实验室突变株肽聚糖组成的剧烈变化。
FEMS Microbiol Lett. 1995 Jul 15;130(1):31-5. doi: 10.1111/j.1574-6968.1995.tb07694.x.
3
Inhibition of the binding of penicillin to the pneumococcal penicillin-binding proteins (PBPs) by exogenous cell wall peptides.外源性细胞壁肽对青霉素与肺炎球菌青霉素结合蛋白（PBPs）结合的抑制作用。
J Gen Microbiol. 1989 Mar;135(3):639-43. doi: 10.1099/00221287-135-3-639.
4
The fib locus in Streptococcus pneumoniae is required for peptidoglycan crosslinking and PBP-mediated beta-lactam resistance.肺炎链球菌中的fib基因座是肽聚糖交联和PBP介导的β-内酰胺抗性所必需的。
FEMS Microbiol Lett. 2000 Jul 1;188(1):81-5. doi: 10.1111/j.1574-6968.2000.tb09172.x.
5
The role of penicillin-binding proteins in penicillin resistance of Streptococcus pneumoniae.青霉素结合蛋白在肺炎链球菌青霉素耐药中的作用
Dev Biol Stand. 1995;85:115-23.
6
Acquisition of five high-Mr penicillin-binding protein variants during transfer of high-level beta-lactam resistance from Streptococcus mitis to Streptococcus pneumoniae.在高水平β-内酰胺抗性从缓症链球菌转移至肺炎链球菌的过程中获得五种高分子量青霉素结合蛋白变体。
J Bacteriol. 1998 Apr;180(7):1831-40. doi: 10.1128/JB.180.7.1831-1840.1998.
7
Interaction of beta-lactam antibiotics with the penicillin-binding proteins of penicillin-resistant Streptococcus pneumoniae.β-内酰胺类抗生素与耐青霉素肺炎链球菌青霉素结合蛋白的相互作用。
Chemotherapy. 1995 May-Jun;41(3):159-64. doi: 10.1159/000239338.
8
Effects of growth of methicillin-resistant and -susceptible Staphylococcus aureus in the presence of beta-lactams on peptidoglycan structure and susceptibility to lytic enzymes.耐甲氧西林和甲氧西林敏感金黄色葡萄球菌在β-内酰胺类药物存在下生长对肽聚糖结构及对溶菌酶敏感性的影响。
Antimicrob Agents Chemother. 1986 Feb;29(2):250-7. doi: 10.1128/AAC.29.2.250.
9
Non-Penicillin-Binding protein mediated high-level penicillin and cephalosporin resistance in a Hungarian clone of Streptococcus pneumoniae.匈牙利肺炎链球菌克隆株中由非青霉素结合蛋白介导的高水平青霉素和头孢菌素耐药性
Microb Drug Resist. 2000 Summer;6(2):105-10. doi: 10.1089/107662900419401.
10
Cross-linking and O-acetylation of peptidoglycan in Staphylococcus aureus (strains H and MR-1) grown in the presence of sub-growth-inhibitory concentrations of beta-lactam antibiotics.在亚生长抑制浓度的β-内酰胺抗生素存在下生长的金黄色葡萄球菌（菌株H和MR-1）中肽聚糖的交联和O-乙酰化
J Gen Microbiol. 1991 Jul;137(7):1661-6. doi: 10.1099/00221287-137-7-1661.
引用本文的文献
1
Biochemical exploration of β-lactamase inhibitors.β-内酰胺酶抑制剂的生化探索
Front Genet. 2023 Jan 17;13:1060736. doi: 10.3389/fgene.2022.1060736. eCollection 2022.
2
The Cell Wall of . 的细胞壁。
Microbiol Spectr. 2019 May;7(3). doi: 10.1128/microbiolspec.GPP3-0018-2018.
3
Rapid antimicrobial susceptibility testing and β-lactam-induced cell morphology changes of Gram-negative biological threat pathogens by optical screening.光学筛选快速检测革兰氏阴性生物威胁病原体的抗生素药敏性和β-内酰胺诱导的细胞形态变化。
BMC Microbiol. 2018 Dec 18;18(1):218. doi: 10.1186/s12866-018-1347-9.
4
Antibiotic-Induced Cell Chaining Triggers Pneumococcal Competence by Reshaping Quorum Sensing to Autocrine-Like Signaling.抗生素诱导的细胞链触发肺炎球菌通过重塑群体感应为自分泌样信号而产生竞争能力。
Cell Rep. 2018 Nov 27;25(9):2390-2400.e3. doi: 10.1016/j.celrep.2018.11.007.
5
In vitro activity and in vivo efficacy of clavulanic acid against Acinetobacter baumannii.克拉维酸对鲍曼不动杆菌的体外活性及体内疗效
Antimicrob Agents Chemother. 2009 Oct;53(10):4298-304. doi: 10.1128/AAC.00320-09. Epub 2009 Jul 27.
6
New formulations of amoxicillin/clavulanic acid: a pharmacokinetic and pharmacodynamic review.阿莫西林/克拉维酸的新制剂：药代动力学与药效学综述
Clin Pharmacokinet. 2005;44(11):1097-115. doi: 10.2165/00003088-200544110-00001.
7
High-level (beta)-lactam resistance and cell wall synthesis catalyzed by the mecA homologue of Staphylococcus sciuri introduced into Staphylococcus aureus.高水平β-内酰胺抗性以及由引入金黄色葡萄球菌的松鼠葡萄球菌mecA同源物催化的细胞壁合成。
J Bacteriol. 2005 Oct;187(19):6651-8. doi: 10.1128/JB.187.19.6651-6658.2005.
8
RWJ-54428 (MC-02,479), a new cephalosporin with high affinity for penicillin-binding proteins, including PBP 2a, and stability to staphylococcal beta-lactamases.RWJ - 54428（MC - 02479），一种对包括PBP 2a在内的青霉素结合蛋白具有高亲和力且对葡萄球菌β - 内酰胺酶稳定的新型头孢菌素。
Antimicrob Agents Chemother. 2003 Feb;47(2):658-64. doi: 10.1128/AAC.47.2.658-664.2003.
9
Effects of specific antibodies against Streptococcus pneumoniae on pharmacodynamic parameters of beta-lactams in a mouse sepsis model.抗肺炎链球菌特异性抗体对小鼠脓毒症模型中β-内酰胺类药物药效学参数的影响
Antimicrob Agents Chemother. 2002 May;46(5):1340-4. doi: 10.1128/AAC.46.5.1340-1344.2002.
10
In vitro activities of oral beta-lactams at concentrations achieved in humans against penicillin-susceptible and -resistant pneumococci and potential to select resistance.口服β-内酰胺类药物在人体所能达到的浓度下对青霉素敏感和耐药肺炎链球菌的体外活性及产生耐药性的可能性。
Antimicrob Agents Chemother. 1998 Aug;42(8):1973-9. doi: 10.1128/AAC.42.8.1973.
本文引用的文献
1
A study of the genetic material determining an enzyme in Pneumococcus.一项关于决定肺炎球菌中一种酶的遗传物质的研究。
Biochim Biophys Acta. 1960 Apr 22;39:508-18. doi: 10.1016/0006-3002(60)90205-5.
2
Penicillin and beyond: evolution, protein fold, multimodular polypeptides, and multiprotein complexes.青霉素及其他：进化、蛋白质折叠、多模块多肽和多蛋白复合物。
Microb Drug Resist. 1996 Summer;2(2):163-75. doi: 10.1089/mdr.1996.2.163.
3
Naturally occurring peptidoglycan variants of Streptococcus pneumoniae.肺炎链球菌的天然存在的肽聚糖变体。
J Bacteriol. 1996 Jan;178(1):168-74. doi: 10.1128/jb.178.1.168-174.1996.
4
The role of penicillin-binding protein 3 (PBP 3) in cefotaxime resistance in Streptococcus pneumoniae.青霉素结合蛋白3（PBP 3）在肺炎链球菌对头孢噻肟耐药中的作用。
Mol Gen Genet. 1993 May;239(1-2):77-80. doi: 10.1007/BF00281604.
5
Triggering of pneumococcal autolysis by lysozyme.溶菌酶引发肺炎球菌自溶
J Infect Dis. 1993 Mar;167(3):684-90. doi: 10.1093/infdis/167.3.684.
6
Purification of penicillin-binding protein 3 from Streptococcus pneumoniae.从肺炎链球菌中纯化青霉素结合蛋白3
Eur J Biochem. 1982 Oct;127(2):231-6. doi: 10.1111/j.1432-1033.1982.tb06860.x.
7
Antibacterial activity of ticarcillin in the presence of clavulanate potassium.替卡西林在克拉维酸钾存在下的抗菌活性。
Am J Med. 1985 Nov 29;79(5B):13-24. doi: 10.1016/0002-9343(85)90124-x.
8
Interaction of non-lytic beta-lactams with penicillin-binding proteins in Streptococcus pneumoniae.非溶菌性β-内酰胺类药物与肺炎链球菌中青霉素结合蛋白的相互作用。
J Gen Microbiol. 1987 Mar;133(3):755-60. doi: 10.1099/00221287-133-3-755.
9
Penicillin-binding protein families: evidence for the clonal nature of penicillin resistance in clinical isolates of pneumococci.青霉素结合蛋白家族：肺炎球菌临床分离株中青霉素耐药性克隆性质的证据。
J Infect Dis. 1989 Jan;159(1):16-25. doi: 10.1093/infdis/159.1.16.
10
Structure of the peptide network of pneumococcal peptidoglycan.肺炎球菌肽聚糖的肽网络结构
J Biol Chem. 1987 Nov 15;262(32):15400-5.
Zotero 插件