Selakovitch-Chenu L, Seroude L, Sicard A M
Laboratoire de Microbiologie et Génétique Moléculaire du CNRS, Toulouse, France.
Mol Gen Genet. 1993 May;239(1-2):77-80. doi: 10.1007/BF00281604.
A pneumococcal strain, with a reduced amount of penicillin-binding protein 3 (PBP 3), permitted an analysis of the role of this protein in cefotaxime resistance. We observed that reduced amounts of PBP 3 sensitize the bacteria to high temperature, to excess glycine and to some D-amino acids. These phenotypes suggest that the amount of PBP 3 may influence the membrane properties of the bacteria. The strain with reduced PBP 3 was transformed to cefotaxime resistance. We show that the PBP 3 mutation, in certain genetic backgrounds, decreases the level of resistance to cefotaxime by a factor of 2. Models are presented to explain this result.
一株肺炎球菌，其青霉素结合蛋白3（PBP 3）含量减少，这使得对该蛋白在头孢噻肟耐药性中的作用进行分析成为可能。我们观察到，PBP 3含量减少会使细菌对高温、过量甘氨酸和某些D - 氨基酸敏感。这些表型表明PBP 3的含量可能会影响细菌的膜特性。PBP 3含量减少的菌株转变为对头孢噻肟耐药。我们表明，在某些遗传背景下，PBP 3突变会使对头孢噻肟的耐药水平降低2倍。文中给出了模型来解释这一结果。
