Brahm H, Piehl-Aulin K, Saltin B, Ljunghall S
Department of Internal Medicine, University Hospital, Uppsala, Sweden.
Calcif Tissue Int. 1997 Feb;60(2):175-80. doi: 10.1007/s002239900210.
The purpose of this study was to evaluate the responses of hormones, growth factors, and biomarkers involved in bone and muscle metabolism during exercise and in recovery. One leg knee-extension exercise and concomitant sampling from the artery and vein were performed. In 12 healthy individuals (6 men and 6 women; age 21-36 years) blood was drawn from the femoral artery and vein at rest, after 10 minutes warm-up, after 15 minutes work at 61% of peak one leg VO2, and after 5 minutes work at peak one leg VO2, as well as 5, 30, and 60 minutes in recovery. Blood flow in the femoral vein was measured using the thermodilution technique. Arteriovenous differences were measured over working thigh for growth hormone (GH), insulin-like growth factor I (IGF-I), insulin-like growth factor binding protein 3 (IGF BP3), parathyroid hormone (PTH) and bone biomarkers, i.e., the carboxyterminal propeptide of type I procollagen (PICP), the carboxyterminal cross-linked telopeptide of type I collagen (ICTP), osteocalcin, and bone-specific alkaline phosphatase (b-ALP). There was an uptake of GH (3.1 +/- 1.2 mU x min(-1), P < 0.001; mean +/- SE) over thigh during exercise and a release of IGF-I at the end of exercise (60 +/- 36 microg x min(-1); P < 0.01). PICP was also released after the maximal exercise (23 +/- 12 microg x min(-1); P < 0.01) as well as ICTP (0.5 +/- 0.3 microg x min(-1); P < 0.05) and b-ALP (0.2 +/- 0.1 microkat x min(-1); P < 0.05). Osteocalcin, IGF BP3, and PTH revealed no clearcut pattern. In the present study, exercise induces endocrine changes which point to anabolic effects on muscle and bone tissue.
本研究的目的是评估运动期间及恢复过程中参与骨骼和肌肉代谢的激素、生长因子及生物标志物的反应。进行了单腿伸膝运动并同时从动脉和静脉采集样本。在12名健康个体（6名男性和6名女性；年龄21 - 36岁）中，于静息状态、热身10分钟后、以单腿峰值摄氧量的61%工作15分钟后、以单腿峰值摄氧量工作5分钟后以及恢复过程中的5分钟、30分钟和60分钟时，从股动脉和股静脉采血。使用热稀释技术测量股静脉血流量。测量工作侧大腿的生长激素（GH）、胰岛素样生长因子I（IGF - I）、胰岛素样生长因子结合蛋白3（IGF BP3）、甲状旁腺激素（PTH）及骨生物标志物，即I型前胶原羧基末端前肽（PICP）、I型胶原羧基末端交联端肽（ICTP）、骨钙素和骨特异性碱性磷酸酶（b - ALP）的动静脉差值。运动期间大腿有GH摄取（3.1±1.2 mU·min⁻¹，P < 0.001；均值±标准误），运动结束时IGF - I释放（60±36 μg·min⁻¹；P < 0.01）。最大运动后PICP也有释放（23±12 μg·min⁻¹；P < 0.01），ICTP（0.5±0.3 μg·min⁻¹；P < 0.05）和b - ALP（0.2±0.1 μkat·min⁻¹；P < 0.05）也有释放。骨钙素、IGF BP3和PTH未显示出明确的变化模式。在本研究中，运动诱导了内分泌变化，表明对肌肉和骨组织有合成代谢作用。
