Thompson K C, Todorovic R A, Hidalgo R J
Res Vet Sci. 1977 Jul;23(1):51-4.
The purpose of the study was to determine whether antigenic differences occurred in four stabilates of Babesia bigemina derived from a single purified isolate and propagated as acute and chronic, blood-borne and tick-borne infections in Colombian cattle. Antigens were characterised by means of the complement fixation (CF), gel diffusion (GD), agar gel electrophoresis (AGE) and the indirect haemagglutination tests (IHA). Differences were detected. Acute blood and chronic blood antigens were similar, as were acute tick and chronic tick antigens, when compared by IHA and GD. Similarities were observed between acute blood anc acute tick and between chronic blood and chronic tick when these antigens were compared by AGE and CF.
本研究的目的是确定源自单一纯化分离株并在哥伦比亚牛中作为急性和慢性、血源和蜱传感染进行繁殖的四种双芽巴贝斯虫稳定株是否存在抗原差异。通过补体结合（CF）、凝胶扩散（GD）、琼脂凝胶电泳（AGE）和间接血凝试验（IHA）对抗原进行了表征。检测到了差异。通过IHA和GD比较时，急性血液和慢性血液抗原相似，急性蜱和慢性蜱抗原也相似。当通过AGE和CF比较这些抗原时，在急性血液和急性蜱之间以及慢性血液和慢性蜱之间观察到了相似性。
