Bonneau C, Couderc R, Tissot M, Athias A, Roch-Arveiller M, Giroud J P
Laboratoire de Pharmacologie, Unité CNRS 15-35, Hôpital Cochin, Paris, France.
Eur J Clin Chem Clin Biochem. 1997 Feb;35(2):73-80. doi: 10.1515/cclm.1997.35.2.73.
Neutrophils play a major role in the host defence by producing reactive oxygen species. These products are liberated by activated cells and are known to cause endothelial cell injury and damage. The present study shows that low-density lipoproteins increase superoxide anion production by twofold in polymorphonuclear leukocytes stimulated by formyl-Met-Leu-Phe in vitro. Moreover, LDL induced a large increase in phosphoinositides and cytosolic-free calcium. Data from experiments performed on neutrophils treated with pertussis toxin, staurosporine, propranolol or niflumic acid suggest that modulation of phospholipase D and A2 activities could be involved in the modification by LDL of leukocyte response to formyl-Met-Leu-Phe. LDL lipid moiety could play a key role in their action on polymorphonuclear functions because cholesterol was exchanged between lipoproteins and cells that can modify membrane fluidity and interact with the formyl-Met-Leu-Phe receptor.
中性粒细胞通过产生活性氧物质在宿主防御中发挥主要作用。这些产物由活化细胞释放，已知会导致内皮细胞损伤。本研究表明，在体外由甲酰甲硫氨酰亮氨酰苯丙氨酸刺激的多形核白细胞中，低密度脂蛋白使超氧阴离子的产生增加了两倍。此外，低密度脂蛋白导致磷酸肌醇和胞质游离钙大幅增加。用百日咳毒素、星形孢菌素、普萘洛尔或氟尼酸处理中性粒细胞的实验数据表明，磷脂酶D和A2活性的调节可能参与了低密度脂蛋白对白细胞由甲酰甲硫氨酰亮氨酰苯丙氨酸引起的反应的修饰。低密度脂蛋白的脂质部分可能在其对多形核功能的作用中起关键作用，因为胆固醇在脂蛋白和细胞之间交换，这会改变膜流动性并与甲酰甲硫氨酰亮氨酰苯丙氨酸受体相互作用。
