Scaccini C, Jialal I
Center for Human Nutrition, University of Texas Southwestern Medical Center at Dallas 75235-9052.
Free Radic Biol Med. 1994 Jan;16(1):49-55. doi: 10.1016/0891-5849(94)90242-9.
The purpose of this study was to determine the most potent activator of the respiratory burst of polymorphonuclear leukocytes (PMN) with respect to the oxidative modification of low density lipoprotein (LDL). Phorbol-12-myristate-13-acetate (PMA), n-formyl-methionyl-leucylphenylalanine (nFMLP), lipopolysaccharide (LPS), and opsonized zymosan (OZ) were tested. The generation of reactive oxygen species by PMN was assayed as superoxide anion production. Oxidative modification of LDL was monitored by thiobarbituric acid reactive substance (TBARS) activity, by conjugated dienes formation at 234nm and by electrophoretic mobility on agarose gel. PMA was the most potent activator of PMN, inducing a 6-fold increase in the superoxide anion production, followed by OZ (3-fold increase). PMA activation also induced the greatest modification of LDL by PMN: 700% increase of conjugated dienes formation, 222% increase of TBARS, and 70% increase in the electrophoretic mobility. The indices of oxidative modification significantly correlated with the superoxide anion generated by different activators. Also, LDL oxidation by PMN was inhibited by superoxide dismutase but not by catalase, methionine, or hydroxyl radical scavengers. Our data indicate that PMNs activated by PMA produce a mildly oxidized form of LDL by a mechanism that appears to involve the superoxide anion.
本研究的目的是确定就低密度脂蛋白（LDL）的氧化修饰而言，多形核白细胞（PMN）呼吸爆发的最有效激活剂。测试了佛波醇-12-肉豆蔻酸酯-13-乙酸酯（PMA）、N-甲酰甲硫氨酰亮氨酰苯丙氨酸（nFMLP）、脂多糖（LPS）和调理酵母聚糖（OZ）。通过超氧阴离子产生来测定PMN产生的活性氧。通过硫代巴比妥酸反应物质（TBARS）活性、234nm处共轭二烯的形成以及琼脂糖凝胶上的电泳迁移率来监测LDL的氧化修饰。PMA是PMN最有效的激活剂，可使超氧阴离子产生增加6倍，其次是OZ（增加3倍）。PMA激活还诱导PMN对LDL的最大修饰：共轭二烯形成增加700%，TBARS增加222%，电泳迁移率增加70%。氧化修饰指标与不同激活剂产生的超氧阴离子显著相关。此外，PMN对LDL的氧化被超氧化物歧化酶抑制，但不被过氧化氢酶、蛋氨酸或羟基自由基清除剂抑制。我们的数据表明，PMA激活的PMN通过一种似乎涉及超氧阴离子的机制产生轻度氧化形式的LDL。
