Todhunter R J, Fubini S L, Freeman K P, Lust G
Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
J Am Vet Med Assoc. 1997 Feb 1;210(3):369-74.
To determine whether keratan sulfate concentrations in plasma or synovial fluid from clinically normal horses were different from concentrations in horses with joint disease and whether concentrations varied with type of joint disease.
Case-control study.
67 clinically normal horses, 10 clinically normal foals, and 160 horses with joint disease.
ELISA was used to measure keratan sulfate concentrations.
Mean plasma keratan sulfate concentration (mean +/- SEM, 580 +/- 124 ng/ml) in foals peaked at 10 weeks of age. Mean plasma keratan sulfate concentration in clinically normal horses was 200 ng/ml (95% confidence interval, 157 to 251 ng/ml). Horses with osteochondral (chip) fractures, other closed intraarticular fractures, inflammatory arthritis (synovitis), infectious arthritis, or osteochondrosis had significantly higher plasma keratan sulfate concentrations than did clinically normal horses, but horses with osteoarthritis did not. Breed, gender, and type of joint disease affected keratan sulfate concentration in synovial fluid. Standard-breds with chip fractures of the metacarpophalangeal/ metatarsophalangeal joints had significantly higher keratan sulfate concentrations in synovial fluid than did Thoroughbreds. Keratan sulfate concentrations in synovial fluid from osteoarthritic carpal joints were lower than concentrations in normal carpal joints and tarsocrural joints with inflammatory joint disease.
Keratan sulfate concentration alone was not a specific marker of joint disease but was affected by various joint diseases.
确定临床正常马匹血浆或滑液中硫酸角质素的浓度是否与患有关节疾病的马匹不同，以及浓度是否随关节疾病类型而变化。
病例对照研究。
67匹临床正常马匹、10匹临床正常马驹和160匹患有关节疾病的马匹。
采用酶联免疫吸附测定法测量硫酸角质素浓度。
马驹血浆中硫酸角质素平均浓度（均值±标准误，580±124纳克/毫升）在10周龄时达到峰值。临床正常马匹血浆中硫酸角质素平均浓度为200纳克/毫升（95%置信区间，157至251纳克/毫升）。患有骨软骨（碎片）骨折、其他闭合性关节内骨折、炎性关节炎（滑膜炎）、感染性关节炎或骨软骨病的马匹血浆中硫酸角质素浓度显著高于临床正常马匹，但患骨关节炎的马匹则不然。品种、性别和关节疾病类型影响滑液中硫酸角质素浓度。掌指关节/跖趾关节有碎片骨折的标准bred马滑液中硫酸角质素浓度显著高于纯种马。患骨关节炎的腕关节滑液中硫酸角质素浓度低于正常腕关节和患有炎性关节疾病的跗关节。
仅硫酸角质素浓度不是关节疾病的特异性标志物，但受多种关节疾病影响。
