Safieddine S, Prior A M, Eybalin M
INSERM U. 254, CHU Hôpital St Charles, Montpellier, France.
Eur J Neurosci. 1997 Feb;9(2):356-67. doi: 10.1111/j.1460-9568.1997.tb01405.x.
The lateral efferent (olivocochlear) innervation of the cochlea originates in the brainstem lateral superior olive. It is likely to use acetylcholine, gamma-aminobutyric acid, dopamine and various neuropeptides as neurotransmitters and/or neuromodulators. In order to determine the different coexistence patterns of these molecules in lateral efferent perikarya, we have used double and triple immunofluorescence co-localization techniques to colocalize choline acetyltransferase, glutamate decarboxylase, tyrosine hydroxylase, calcitonin gene-related peptide and enkephalins in single sections of the lateral superior olive. We also used a non-radioactive in situ hybridization technique onto serial sections of this nucleus to confirm the immunofluorescence co-localization data at the mRNA level. Whatever the pair or triplet of primary antibodies tested was, a high ratio of coexistence was observed in the immunofluorescence experiments. In triple co-localization experiments, 90-93% of the choline acetyltransferase-like immunoreactive neurons were also immunoreactive to the two other antigens investigated. The in situ hybridization co-localization data, based on the use of biotin-labelled oligoprobes, qualitatively confirmed these immunofluorescence data. In conclusion, it can be postulated that acetylcholine, gamma-aminobutyric acid, dopamine, calcitonin gene-related peptide, enkephalins and dynorphins (whose coexistence with choline acetyltransferase and enkephalins has been previously described immunocytochemically) coexist in lateral efferent neurons. Based on these results, it is tempting to propose the lateral efferent innervation as a useful model with which the functional implications of the coexistence of neurotransmitters/neuromodulators can be investigated in vivo.
耳蜗的外侧传出（橄榄耳蜗）神经支配起源于脑干的外侧上橄榄核。它可能使用乙酰胆碱、γ-氨基丁酸、多巴胺和各种神经肽作为神经递质和/或神经调质。为了确定这些分子在外侧传出神经元胞体中的不同共存模式，我们使用了双重和三重免疫荧光共定位技术，在外侧上橄榄核的单个切片中对胆碱乙酰转移酶、谷氨酸脱羧酶、酪氨酸羟化酶、降钙素基因相关肽和脑啡肽进行共定位。我们还对该核的连续切片使用了非放射性原位杂交技术，以在mRNA水平上确认免疫荧光共定位数据。无论测试的一抗对或三联体是什么，在免疫荧光实验中都观察到了高比例的共存。在三重共定位实验中，90 - 93%的胆碱乙酰转移酶样免疫反应性神经元对其他两种研究抗原也有免疫反应性。基于生物素标记寡核苷酸探针的原位杂交共定位数据在质量上证实了这些免疫荧光数据。总之，可以推测乙酰胆碱、γ-氨基丁酸、多巴胺、降钙素基因相关肽、脑啡肽和强啡肽（其与胆碱乙酰转移酶和脑啡肽的共存先前已通过免疫细胞化学方法描述）在外侧传出神经元中共存。基于这些结果，很诱人地提出外侧传出神经支配作为一个有用的模型，通过它可以在体内研究神经递质/神经调质共存的功能意义。
