Wahr J A, Anderson M M, Giacherio D A, Hallock L, Gawryl M S, Lansden C, Tremper K K
Department of Anesthesiology, University of Michigan Medical Center, Ann Arbor, USA.
J Cardiothorac Vasc Anesth. 1997 Feb;11(1):10-2. doi: 10.1016/s1053-0770(97)90244-5.
Hemoglobin-based oxygen carriers will be used concurrently with intraoperative blood salvage. The effects of salvage and processing on blood containing one such solution (HBOC-201; Biopure Corp, Boston, MA) were studied.
Prospective, randomized.
Laboratory.
Sixteen blood units from healthy volunteers had either HBOC (1,500 mg/dL; n = 10) or normal saline (equivalent volume; n = 6) added. All units were salvaged and processed using a blood salvage device. Samples were analyzed for the concentration and molecular weight distribution of plasma hemoglobin and red cell morphology presalvage (pre) and following processing and washing (post 1). Five of the HBOC units underwent a second 1,000 mL wash (post 2).
Processing and washing decreased the concentration of plasma hemoglobin (mg/dL) in HBOC units (1311 +/- 265 pre to 27.8 +/- 19.6 post 1 to 6.5 +/- 2.19 post 2), but did not change the plasma hemoglobin concentration in saline units (2.05 +/- 1.27 pre v 3.18 +/- 0.79 post 1). Total plasma hemoglobin in HBOC units (6.56 +/- 2.19) was significantly greater than in saline units (3.18 +/- 0.79), even after the second wash (post 2). The concentration of unstable hemoglobin in the plasma phase was not different between groups. Red cell morphology was altered by the salvage process but was not different between groups.
Salvage and processing of blood containing HBOC yield concentrated red cells that are indistinguishable from those obtained from blood without HBOC. Residual HBOC remains but is unchanged from the HBOC initially administered.
基于血红蛋白的氧载体将与术中血液回收同时使用。研究了回收和处理对含有一种此类溶液（HBOC-201；Biopure公司，马萨诸塞州波士顿）的血液的影响。
前瞻性、随机对照。
实验室。
向来自健康志愿者的16个血液单位中加入HBOC（1500mg/dL；n = 10）或生理盐水（等体积；n = 6）。所有单位均使用血液回收装置进行回收和处理。分析样品在回收前（pre）以及处理和洗涤后（post 1）的血浆血红蛋白浓度和分子量分布以及红细胞形态。5个HBOC单位进行了第二次1000mL洗涤（post 2）。
处理和洗涤降低了HBOC单位中血浆血红蛋白的浓度（mg/dL）（回收前为1311±265，post 1为27.8±19.6，post 2为6.5±2.19），但未改变生理盐水单位中血浆血红蛋白的浓度（回收前为2.05±1.27，post 1为3.18±0.79）。即使在第二次洗涤后（post 2），HBOC单位中的总血浆血红蛋白（6.56±2.19）仍显著高于生理盐水单位（3.18±0.79）。血浆相中不稳定血红蛋白的浓度在各组之间没有差异。红细胞形态因回收过程而改变，但各组之间没有差异。
含有HBOC的血液的回收和处理产生的浓缩红细胞与不含HBOC的血液所获得的红细胞无法区分。残留的HBOC仍然存在，但与最初给予的HBOC没有变化。
