Kavookjian A M, Brown R M
Cytobios. 1978;23(89):45-70.
The cell wall of the marine alga Pleurochrysis scherffelii is composed of Golgi-derived scales with four distinct subcomponents: amorphous coating material, radial microfibrils, spiral coating substances and spiral microfibrils. The antigenic properties of the Pleurochrysis scales were studied. Antibodies were produced in rabbits to the amorphour to the amorphous coating material and to a heretofore undescribed peripheral subcompoent, known as the amorphoous rim modification substance. The antigenic subcomponents were detected by agglutination/precipitation, negative staining, and shadowing procedures. Scales of the Cricosphaera phase produced negative results when tested with Pleurochrysis scale antisera. A possible function of the amorphous rim modification substance in maintaining the integrity of spiral microfibrillar organization is introduced.
海洋藻类施氏侧金盏藻的细胞壁由高尔基体衍生的鳞片组成,这些鳞片有四个不同的亚成分:无定形涂层物质、放射状微纤维、螺旋状涂层物质和螺旋状微纤维。对施氏侧金盏藻鳞片的抗原特性进行了研究。用兔子制备了针对无定形涂层物质和一种迄今未描述的外周亚成分(称为无定形边缘修饰物质)的抗体。通过凝集/沉淀、负染色和投影法检测抗原亚成分。用施氏侧金盏藻鳞片抗血清检测时,球石藻阶段的鳞片产生阴性结果。介绍了无定形边缘修饰物质在维持螺旋状微纤维组织完整性方面可能的功能。