Melkonian M, Becker B, Becker D
Universität zu Köln, Botanisches Institut, Federal Republic of Germany.
J Electron Microsc Tech. 1991 Feb;17(2):165-78. doi: 10.1002/jemt.1060170205.
Scale biogenesis in algae represents a unique model system to study the transport of secretory macromolecules through the Golgi apparatus (GA) and their exocytosis. The larger scales can be visualized in the light microscope, and thus the kinetics of scale assembly, transport, and secretion can be studied in vivo. In addition, scales are osmiophilic and readily visible in conventional transmission electron microscopy; thus, details of scale assembly and sorting can be studied without invoking immunolabeling techniques. The following are distinctive features of scale biogenesis in algae: 1) transport of scales through the GA-stack occurs by cisternal progression; 2) scale secretion may be very rapid (in some cases a single GA-cisterna leaves the stack every 15-20 s); 3) sorting of different scale types does not occur in the GA, but in a post-GA-compartment. Recent progress in the analysis of scale formation in the green flagellates Tetraselmis and Scherffelia is reviewed.
藻类中的鳞片生物发生代表了一个独特的模型系统，用于研究分泌性大分子通过高尔基体（GA）的运输及其胞吐作用。较大的鳞片可以在光学显微镜下观察到，因此可以在体内研究鳞片组装、运输和分泌的动力学。此外，鳞片具有嗜锇性，在传统透射电子显微镜下很容易看到；因此，无需借助免疫标记技术就可以研究鳞片组装和分选的细节。以下是藻类鳞片生物发生的显著特征：1）鳞片通过高尔基体堆叠的运输是通过潴泡进展进行的；2）鳞片分泌可能非常迅速（在某些情况下，每15-20秒就有一个高尔基体潴泡离开堆叠）；3）不同类型鳞片的分选不是在高尔基体中进行，而是在高尔基体后区室中进行。本文综述了绿藻鞭毛虫Tetraselmis和Scherffelia中鳞片形成分析的最新进展。
