Woo J T, Ohba Y, Tagami K, Sumitani K, Kataoka T, Nagai K
Department of Bioengineering, Tokyo Institute of Technology, Kanagawa, Japan.
Biosci Biotechnol Biochem. 1997 Feb;61(2):400-2. doi: 10.1271/bbb.61.400.
Prodigiosin 25-C and metacycloprodigiosin were found to suppress PTH-stimulated pit formation by cultured osteoclasts on bone slices. They also inhibited the acidification of vacuolar organelles in intact osteoclastic cells. Since the acidic pH in these organelles is generated by the action of proton-pumping ATPases of the organelle, these results indicate that the proton-pumping activity of V-ATPase in osteoclastic cells is essential in bone resorption and that the inhibition of the acidification of vacuolar organelles by prodigiosins results in suppression of PTH-stimulated bone resorption.
研究发现，灵菌红素25-C和间环灵菌红素可抑制培养的破骨细胞在骨切片上形成甲状旁腺激素刺激的小窝。它们还抑制完整破骨细胞中液泡细胞器的酸化。由于这些细胞器中的酸性pH是由细胞器的质子泵ATP酶的作用产生的，这些结果表明破骨细胞中V-ATP酶的质子泵活性在骨吸收中至关重要，并且灵菌红素对液泡细胞器酸化的抑制导致甲状旁腺激素刺激的骨吸收受到抑制。
