Ludwig L L, Schertel E R, Pratt J W, McClure D E, Ying A J, Heck C F, Myerowitz P D
Department of Surgery, Ohio State University, Columbus 43210, USA.
Cardiovasc Res. 1997 Jan;33(1):164-71. doi: 10.1016/s0008-6363(96)00177-0.
We performed the following study to define the effects of acute cardiac lymphatic obstruction on left ventricular (LV) systolic and diastolic function.
Cardiac lymphatic obstruction was created in 8 pentobarbital-anesthetized dogs by identifying (Evans blue) and ligating the right and left epicardial lymphatics, the afferent and efferent lymphatics associated with the pretrachael and cardiac lymph nodes, and the thoracic duct. Left ventricular function was assessed by analysis of micromanometer-conductance catheter-derived LV pressure-volume relationships. Contractility was assessed by preload recruitable stroke work (PRSW). The active and passive phases of LV relaxation were assessed by the time constant o isovolumic relaxation (tau) and the end-diastolic pressure-volume relationship (stiffness), respectively.
PRSW decreased significantly and tau increased significantly from baseline at 1, 2, and 3 h after cardiac lymphatic obstruction (n = 8), but stiffness did not change. Cardiac lymphatic obstruction had similar effects on LV function in a group of autonomically blocked dogs (n = 5). Left ventricular function did not change in sham treated controls (n = 8). Cardiac lymphatic obstruction induced a significant increase in LV wet/dry weight ratios (3.58 +/- 0.01) when compared to the control group (3.53 +/- 0.02). Histopathology of the myocardium in the lymphatic obstruction groups revealed significant lymphangiectasis and increased interstitial spacing when compared to controls.
Acute cardiac lymphatic obstruction depresses contractility and active relaxation and causes mild LV myocardial edema, but does not alter diastolic stiffness.
我们进行了以下研究以确定急性心脏淋巴管阻塞对左心室（LV）收缩和舒张功能的影响。
在8只戊巴比妥麻醉的犬中造成心脏淋巴管阻塞，方法是识别（伊文思蓝）并结扎左右心外膜淋巴管、与气管前和心脏淋巴结相关的输入和输出淋巴管以及胸导管。通过分析微测压-电导导管得出的左心室压力-容积关系来评估左心室功能。通过预负荷可募集搏功（PRSW）评估收缩性。分别通过等容舒张时间常数（tau）和舒张末期压力-容积关系（硬度）评估左心室舒张的主动和被动阶段。
心脏淋巴管阻塞后1、2和3小时，PRSW较基线显著降低，tau显著增加（n = 8），但硬度未改变。心脏淋巴管阻塞对一组自主神经阻断的犬（n = 5）的左心室功能有类似影响。假手术对照组（n = 8）的左心室功能未改变。与对照组相比，心脏淋巴管阻塞导致左心室湿/干重比显著增加（3.58±0.01），而对照组为（3.53±0.02）。与对照组相比，淋巴管阻塞组心肌的组织病理学显示明显的淋巴管扩张和间质间隙增加。
急性心脏淋巴管阻塞会降低收缩性和主动舒张，并导致轻度左心室心肌水肿，但不会改变舒张硬度。
