Doi M, Kasuya H, Weir B, Cook D A, Ogawa A
Department of Neurosurgery, Iwate Medical University, Japan.
Acta Neurochir (Wien). 1997;139(1):77-81. doi: 10.1007/BF01850872.
Calponin, an actin- and tropomyosin-binding protein, has been characterized as an inhibitory factor in the smooth-muscle actomyosin activity. The level of calponin was determined in canine basilar arteries in a double-haemorrhage model. Thirty dogs were assigned to three groups: day 0 group, control; day 2 group, dogs sacrificed 2 days after cisternal injection of blood; and day 7 group, dogs given double cisternal injections of blood and sacrificed 7 days after the first injection. Constriction of the basilar artery was confirmed by arterial angiography. Portions of the affected arteries or the corresponding region in control animals were solubilized for sodium dodecylsulphate-polyacrylamide gel electrophoresis and Western blotting. A major band corresponding to calponin was seen at 34 kD in the basilar artery extracts using chicken gizzard polyclonal antibodies. The densitometer values of the band on Coomassie blue-stained gels were expressed as percentages of day 0 control values. The signals of day 2 and day 7 samples were 47% +/- 20% and 23% +/- 12%, respectively (mean +/- standard deviation). The proportions of calponin to actin/tropomyosin in the day 0, day 2, and day 7 groups were 13% +/- 6%, 6% +/- 2%, and 4% +/- 2%, respectively. The reduced expression of calponin may be related to sustained contraction during cerebral vasospasm.
钙调蛋白是一种与肌动蛋白和原肌球蛋白结合的蛋白质，已被确定为平滑肌肌动球蛋白活性的抑制因子。在双出血模型中测定了犬基底动脉中钙调蛋白的水平。30只狗被分为三组：第0天组，对照组；第2天组，在脑池内注射血液2天后处死的狗；第7天组，接受两次脑池内血液注射并在第一次注射后7天处死的狗。通过动脉血管造影证实基底动脉收缩。将受影响动脉的部分或对照动物的相应区域溶解，用于十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和蛋白质免疫印迹分析。使用鸡肌胃多克隆抗体，在基底动脉提取物中可见一条对应于钙调蛋白的主要条带，位于34 kD处。考马斯亮蓝染色凝胶上条带的光密度计值表示为第0天对照值的百分比。第2天和第7天样本的信号分别为47%±20%和23%±12%（平均值±标准差）。第0天、第2天和第7天组中钙调蛋白与肌动蛋白/原肌球蛋白的比例分别为13%±6%、6%±2%和4%±2%。钙调蛋白表达降低可能与脑血管痉挛期间的持续收缩有关。
