Contiero E, Ferrari R, Vaselli G M, Folin M
Department of Biology, Camposampiero Hospital, Padua, Italy.
Electrophoresis. 1997 Jan;18(1):122-6. doi: 10.1002/elps.1150180123.
Quantitative evaluation of serum apolipoprotein AI (apo AI) isoforms through densitometric analysis following isoelectric focusing (IEF) is described. The apo AI isoforms were identified by immunoblotting. By combining these techniques, a qualitatively invariant pattern was observed in 54 serum samples, obtained from 27 men and 27 women. Peak 1 (proapo AI, pI 5.75) accounts for about 8% of the total densitometric area, peak 3 (apo AIzero, pI 5.59) for about 74%, peak 4 (apo AI-1, pI 5.42) for about 13%, and peak 5 (apo AI-2, pI 5.37) for about 5%. In some subjects, the proportion of proapo AI was increased. Variations of the ratio of the different apo AI isoforms may be due to modification of the fractional catabolic rate of this apolipoprotein. The procedure proposed in this study may be useful for the evaluation of quantitative abnormalities in apo AI isoforms involved in the development of coronary heart diseases (CHD).
本文描述了通过等电聚焦（IEF）后密度分析对血清载脂蛋白AI（apo AI）亚型进行定量评估的方法。通过免疫印迹法鉴定apo AI亚型。结合这些技术，在从27名男性和27名女性获得的54份血清样本中观察到了定性不变的模式。峰1（前载脂蛋白AI，pI 5.75）约占总密度面积的8%，峰3（apo AIzero，pI 5.59）约占74%，峰4（apo AI-1，pI 5.42）约占13%，峰5（apo AI-2，pI 5.37）约占5%。在一些受试者中，前载脂蛋白AI的比例增加。不同apo AI亚型比例的变化可能是由于这种载脂蛋白的分数分解代谢率的改变。本研究中提出的方法可能有助于评估参与冠心病（CHD）发展的apo AI亚型的定量异常。
