Kachroo P, Lee K H, Schwerdel C, Bailey J E, Chattoo B B
Department of Microbiology, Faculty of Science, M. S. University, Baroda, India.
Electrophoresis. 1997 Jan;18(1):163-9. doi: 10.1002/elps.1150180129.
Two-dimensional (2-D) polyacrylamide gel electrophoresis of proteins was used to study the response of the rice blast fungus to extracts prepared from resistant and susceptible rice cultivars. A protein of molecular mass 31 kDa was induced by a susceptible host extract, while the fungus exposed to extract from the resistant cultivar and the untreated samples did not show the presence of this protein. Levels of this 31 kDa protein increased 30-fold, 72 h after treatment with plant extracts, with the concomitant appearance of at least sixteen other novel proteins. Fungus treated with extracts of resistant host or the untreated samples did not show any of these proteins while the proteins specific to different growth stages appeared as expected. Analysis of the extracellular samples showed induction of a 17 kDa protein after 72 h in the culture treated with susceptible host extract. Since the resistant host extract does not cause induction of any protein it is likely that the proteins induced in response to the susceptible host are expressed during the disease process and/or its establishment. Our study demonstrates usefulness of 2-D analysis in understanding host-pathogen interactions.
利用蛋白质二维聚丙烯酰胺凝胶电泳研究稻瘟病菌对感病和抗病水稻品种提取物的反应。一种分子量为31 kDa的蛋白质由感病寄主提取物诱导产生，而暴露于抗病品种提取物和未处理样品中的真菌未显示出该蛋白质的存在。用植物提取物处理72小时后，这种31 kDa蛋白质的水平增加了30倍，同时至少出现了另外16种新蛋白质。用抗病寄主提取物处理的真菌或未处理样品未显示出这些蛋白质中的任何一种，而不同生长阶段特有的蛋白质则如预期出现。对细胞外样品的分析表明，在用感病寄主提取物处理的培养物中，72小时后诱导产生了一种17 kDa的蛋白质。由于抗病寄主提取物不会诱导任何蛋白质，因此很可能响应感病寄主而诱导产生的蛋白质是在病害过程和/或其建立过程中表达的。我们的研究证明了二维分析在理解寄主-病原体相互作用方面的有用性。
