Zhou Haiying, Hwarari Delight, Zhang Yunhui, Mo Xiaosong, Luo Yuming, Ma Hongyu
Jiangsu Key Laboratory for Eco-Agricultural Biotechnology around Hongze Lake, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, Huaiyin Normal University, Huai'an 223300, China.
College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China.
Plants (Basel). 2022 Jun 27;11(13):1702. doi: 10.3390/plants11131702.
Rice blast disease caused by a fungus, , is one of the most destructive diseases in rice production worldwide, and salicylic acid (SA) can efficiently decrease the damage of . Here, we combined the 2-Dimensional-Liquid Chromatography and the Matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (2D-LC-MALDI-TOF-TOF MS) techniques to compare and identify differentially expressed labelled proteins by the isobaric tags for relative and absolute quantitation (iTRAQ) between the blast-resistant cultivar Minghui and the susceptible rice cultivar Nipponbare in response to blast fungus infection. The group samples were treated with salicylic acid and compared to control samples. A total of 139 DEPs from the two cultivars showed either more than a two-fold change or alternating regulation patterns. Protein functionality analysis also exhibited that these proteins are involved in a wide range of molecular functions including: energy-related activity (30%), signal transduction (11%), redox homeostasis (15%), amino acid and nitrogen metabolism (4%), carbohydrate metabolism (5%), protein folding and assembly (10%), protein hydrolysis (9%), protein synthesis (12%), and other unknown functions (4%). Specifically, we demonstrated that exogenous treatment with salicylic acid promoted recovery in both rice cultivars from infection by enhancing: the regulation of signal transduction, increasing energy conversion and production through the regulation of the glycolytic pathway, and other various biochemical processes. These findings may facilitate future studies of the molecular mechanisms of rice blast resistance.
由真菌引起的稻瘟病是全球水稻生产中最具破坏性的病害之一，水杨酸（SA）可以有效减轻其危害。在此，我们结合二维液相色谱和基质辅助激光解吸/电离飞行时间质谱仪（2D-LC-MALDI-TOF-TOF MS）技术，通过相对和绝对定量等压标签（iTRAQ）比较并鉴定了抗稻瘟病品种明恢和感病水稻品种日本晴在稻瘟病菌感染后的差异表达标记蛋白。对样本组进行水杨酸处理，并与对照样本进行比较。两个品种共有139个差异表达蛋白表现出两倍以上的变化或交替调控模式。蛋白质功能分析还表明，这些蛋白质涉及广泛的分子功能，包括：能量相关活性（30%）、信号转导（11%）、氧化还原稳态（15%）、氨基酸和氮代谢（4%）、碳水化合物代谢（5%）、蛋白质折叠和组装（10%）、蛋白质水解（9%）、蛋白质合成（12%）以及其他未知功能（4%）。具体而言，我们证明了水杨酸的外源处理通过增强信号转导调控、通过糖酵解途径调控增加能量转换和产生以及其他各种生化过程，促进了两个水稻品种从感染中恢复。这些发现可能有助于未来对水稻抗稻瘟病分子机制的研究。
