Kasravi F B, Adawi D, Molin G, Bengmark S, Jeppsson B
Department of Surgery, Lund University, Sweden.
J Hepatol. 1997 Feb;26(2):417-24. doi: 10.1016/s0168-8278(97)80060-8.
BACKGROUND/AIM: Bacterial infections and sepsis are frequent complications of acute liver injury, with a high share in the mortality and morbidity of this condition. Bacterial translocation from the gut may play an important role in the high rate of infections observed. In this experiment the effect of different oral supplementation on bacterial translocation was evaluated in acute liver injury induced by D-galactosamine in the rat.
Rats were given oral supplements of lactulose, neomycin, Lactobacillus reuteri R2LC, and Lactobacillus plantarum DSM 9843 for 1 week. Liver injury was induced by intraperitoneal administration of 1.1 g/kg D-galactosamine. Twenty-four hours later, rats were sacrificed and liver enzymes and histology, intestinal bacterial count and microflora, intestinal mucosal histology, DNA and RNA content, bacterial translocation to blood, mesenteric lymph nodes, and liver, and serum endotoxin were studied or measured.
Lactulose was highly effective in prevention of liver injury and bacterial translocation. Neomycin and Lactobacillus plantarum DSM 9843 showed a moderate effect in prevention of liver injury and bacterial translocation. Intestinal bacterial count and microflora were affected by different treatment modalities. No endotoxin concentration was found in any of the experimental groups. Both lactobacilli could significantly improve the mucosal proliferative state.
Oral supplementation of lactulose with anti-endotoxin effect could successfully prevent the liver injury and the subsequent bacterial translocation in acute liver injury induced by administration of D-galactosamine in the rat. This effect was irrespective of the intestinal bacterial alteration or mucosal proliferative state.
背景/目的：细菌感染和败血症是急性肝损伤常见的并发症，在该病症的死亡率和发病率中占比很高。肠道细菌易位可能在观察到的高感染率中起重要作用。在本实验中，评估了不同口服补充剂对D-半乳糖胺诱导的大鼠急性肝损伤中细菌易位的影响。
给大鼠口服乳果糖、新霉素、罗伊氏乳杆菌R2LC和植物乳杆菌DSM 9843补充剂，持续1周。通过腹腔注射1.1 g/kg D-半乳糖胺诱导肝损伤。24小时后，处死大鼠，研究或测量肝酶和组织学、肠道细菌计数和微生物群落、肠黏膜组织学、DNA和RNA含量、细菌向血液、肠系膜淋巴结和肝脏的易位以及血清内毒素。
乳果糖在预防肝损伤和细菌易位方面非常有效。新霉素和植物乳杆菌DSM 9843在预防肝损伤和细菌易位方面显示出中等效果。不同治疗方式会影响肠道细菌计数和微生物群落。在任何实验组中均未发现内毒素浓度。两种乳杆菌均可显著改善黏膜增殖状态。
口服具有抗内毒素作用的乳果糖可成功预防D-半乳糖胺诱导的大鼠急性肝损伤中的肝损伤及随后的细菌易位。这种作用与肠道细菌改变或黏膜增殖状态无关。
