Hiepe F, Jungnitz S, Hiepe T
Institut für Parasitologie und Tropenveterinärmedizin, Freien Universität Berlin.
Appl Parasitol. 1996 Dec;37(4):266-74.
The aim of the study was to investigate the induction of autoantibodies in rabbits after the inoculation with Trypanosoma equiperdum. Eight female rabbits were inoculated s.c. with 5 x 10(6) parasites of Trypanosoma equiperdum. Four of these animals were treated with Diminazen (Berenil) at a dosage of 28 mg per rabbit by intramuscular injection 35 days p. i. Two additional non-infected rabbits served as negative controls. 9 days p.i. all infected animals possessed serum antibodies against Typanosoma equiperdum. Titers peaked to 1:2048, measured between 14 and 30 days p.i. and were persistent until the end of examinations. In parallel, there was an increase of anti-dsDNA and anti-collagen autoantibodies, but not of autoantibodies against recombinant U1RNP and La antigens. In comparison to patients with systemic lupus erythematosus, the anti-DNA binding of rabbit's sera was reduced more strongly in presence of increasing salt concentrations. In addition, the sera of inoculated rabbits recognized several unknown bands of a cell extract in immunoblotting. The parallel increase of antibodies against Trypanosoma, control foreign antigens, DNA and collagen suggests a polyclonal stimulation of the immune system. It was concluded that this rabbit model seems to be sufficient to investigate the mechanisms which are able to induce autoimmune phenomena.
本研究的目的是调查接种马媾疫锥虫后兔体内自身抗体的诱导情况。8只雌性兔经皮下接种5×10⁶个马媾疫锥虫寄生虫。其中4只动物在感染后35天通过肌肉注射以每只兔28mg的剂量用二脒那秦（贝尼尔）进行治疗。另外2只未感染的兔作为阴性对照。感染后9天，所有感染动物均拥有抗马媾疫锥虫的血清抗体。抗体效价在感染后14至30天达到峰值1:2048，并一直持续到检查结束。同时，抗双链DNA和抗胶原蛋白自身抗体有所增加，但抗重组U1RNP和La抗原的自身抗体未增加。与系统性红斑狼疮患者相比，兔血清的抗DNA结合在盐浓度增加时下降得更强烈。此外，接种兔的血清在免疫印迹中识别出细胞提取物的几条未知条带。针对马媾疫锥虫、对照外来抗原、DNA和胶原蛋白的抗体平行增加表明免疫系统受到多克隆刺激。得出的结论是，该兔模型似乎足以研究能够诱导自身免疫现象的机制。
