三链体导致聚合停滞的机制。
Mechanisms of triplex-caused polymerization arrest.
作者信息
Krasilnikov A S, Panyutin I G, Samadashwily G M, Cox R, Lazurkin Y S, Mirkin S M
机构信息
Department of Genetics, University of Illinois at Chicago, Chicago, IL 60607, USA.
出版信息
Nucleic Acids Res. 1997 Apr 1;25(7):1339-46. doi: 10.1093/nar/25.7.1339.
Abstract
Pyrimidine/purine/purine triplexes are known to inhibit DNA polymerization. Here we have studied the mechanisms of this inhibition by comparing the efficiency of Vent DNA polymerase on triplex- and duplex-containing templates at different temperatures, Mg2+concentrations and time intervals with the thermal stability of the corresponding structures. Our results show that triplexes can only be by-passed at temperatures where thermal denaturation initiates, while duplexes, in contrast, are overcome at temperatures where they are quite stable. These results show that DNA polymerase cannot untangle triplex regions within DNA templates and seems to entirely depend on their thermal fluctuations. The high stability of triplexes at physiological temperatures and ambient conditions make them a barrier to polymerization.
摘要
已知嘧啶/嘌呤/嘌呤三链体可抑制DNA聚合反应。在此，我们通过比较Vent DNA聚合酶在不同温度、Mg2+浓度和时间间隔下对含三链体和双链体模板的作用效率以及相应结构的热稳定性，研究了这种抑制作用的机制。我们的结果表明，只有在热变性起始的温度下，三链体才能被绕过，而相比之下，双链体在其相当稳定的温度下就能被克服。这些结果表明，DNA聚合酶无法解开DNA模板内的三链体区域，似乎完全依赖于它们的热涨落。三链体在生理温度和环境条件下的高稳定性使其成为聚合反应的一个障碍。
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