核糖核酸酶YI*与RNA结构研究。
RNase YI* and RNA structure studies.
作者信息
Cannistraro V J, Kennell D
机构信息
Department of Molecular Microbiology, Washington University School of Medicine, St Louis, MO 63110, USA.
出版信息
Nucleic Acids Res. 1997 Apr 1;25(7):1405-12. doi: 10.1093/nar/25.7.1405.
The enzymology of RNase YI*, a recently discovered endoribonuclease from yeast, was studied and compared to other endonucleases for detection of single-strand regions and base pair mismatches in RNA. Its value for RNA structure analyses was assessed with Escherichia coli 5S rRNA as a model substrate. The generally accepted structure of the 5S rRNA is based on thermodynamic energy considerations as well as structures conserved in regions of the molecule during evolution. S1 and mung bean nucleases gave similar results with very marked preference only for the longest single-stranded region in the model. RNase YI* was much more discriminating for detecting unpaired nucleotides as well as short single-strand regions and predicted the generally accepted 5S rRNA structure. Preliminary experiments also indicated that RNase YI* was more sensitive than RNase I for detecting single or multiple base pair mismatches in an RNA-DNA hybrid.
对最近从酵母中发现的核糖核酸酶YI的酶学性质进行了研究，并与其他核酸内切酶进行比较，以检测RNA中的单链区域和碱基对错配。以大肠杆菌5S rRNA作为模型底物评估了其在RNA结构分析中的价值。5S rRNA的普遍接受结构是基于热力学能量考量以及该分子在进化过程中各区域保守的结构。S1核酸酶和绿豆核酸酶给出了相似的结果，且仅对模型中最长的单链区域有非常明显的偏好。核糖核酸酶YI在检测未配对核苷酸以及短单链区域方面更具辨别力，并预测出了普遍接受的5S rRNA结构。初步实验还表明，核糖核酸酶YI*在检测RNA-DNA杂交体中的单碱基或多碱基对错配方面比核糖核酸酶I更敏感。
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