细胞YY1转录因子与爱泼斯坦-巴尔病毒BRLF1启动子中的一个顺式作用负调控元件结合。
The cellular YY1 transcription factor binds a cis-acting, negatively regulating element in the Epstein-Barr virus BRLF1 promoter.
作者信息
Zalani S, Coppage A, Holley-Guthrie E, Kenney S
机构信息
Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, 27599-7295, USA.
出版信息
J Virol. 1997 Apr;71(4):3268-74. doi: 10.1128/JVI.71.4.3268-3274.1997.
Abstract
Disruption of Epstein-Barr virus latency is induced by expression of either the BZLF1 (in B cells and epithelial cells) or BRLF1 (in epithelial cells only) immediate-early protein. Regulation of BZLF1 and BRLF1 transcription may therefore modulate the stringency of viral latency. The cellular transcription factor YY1 negatively regulates BZLF1 transcription. Here we show that the BRLF1 promoter (Rp) sequences from -206 to -227 (relative to the mRNA start site) and from -7 to +6 are directly bound by YY1. Mutation of the upstream YY1 binding site increases constitutive Rp activity in epithelial cells and B cells, while mutation of the downstream YY1 binding site does not significantly affect Rp activity. Negative regulation of BZLF1 and BRLF1 transcription by YY1 may act to maintain viral latency.
摘要
爱泼斯坦-巴尔病毒潜伏期的破坏是由BZLF1（在B细胞和上皮细胞中）或BRLF1（仅在上皮细胞中）立即早期蛋白的表达所诱导的。因此，BZLF1和BRLF1转录的调节可能会调节病毒潜伏期的严格程度。细胞转录因子YY1负向调节BZLF1转录。在这里，我们表明，YY1直接结合BRLF1启动子（Rp）从-206到-227（相对于mRNA起始位点）以及从-7到+6的序列。上游YY1结合位点的突变增加了上皮细胞和B细胞中组成型Rp活性，而下游YY1结合位点的突变对Rp活性没有显著影响。YY1对BZLF1和BRLF1转录的负向调节可能起到维持病毒潜伏期的作用。
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本文引用的文献
1
Epstein-Barr viral latency is disrupted by the immediate-early BRLF1 protein through a cell-specific mechanism.爱泼斯坦-巴尔病毒潜伏状态通过一种细胞特异性机制被即刻早期BRLF1蛋白破坏。
Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):9194-9. doi: 10.1073/pnas.93.17.9194.
2
Evidence for physical interaction between the zinc-finger transcription factors YY1 and Sp1.锌指转录因子YY1和Sp1之间存在物理相互作用的证据。
Proc Natl Acad Sci U S A. 1993 Jul 1;90(13):6145-9. doi: 10.1073/pnas.90.13.6145.
3
Human transcription factor YY1 represses human immunodeficiency virus type 1 transcription and virion production.人类转录因子YY1可抑制1型人类免疫缺陷病毒的转录及病毒体产生。
J Virol. 1994 Feb;68(2):905-10. doi: 10.1128/JVI.68.2.905-910.1994.
4
YY1 is the cellular factor shown previously to bind to regulatory regions of several leaky-late (beta gamma, gamma 1) genes of herpes simplex virus type 1.YY1是先前已证明能与1型单纯疱疹病毒的几个渗漏晚期（βγ、γ1）基因的调控区域结合的细胞因子。
J Virol. 1994 Feb;68(2):1234-8. doi: 10.1128/JVI.68.2.1234-1238.1994.
5
Inhibition of transcriptional regulator Yin-Yang-1 by association with c-Myc.通过与c-Myc结合抑制转录调节因子阴阳-1
Science. 1993 Dec 17;262(5141):1889-92. doi: 10.1126/science.8266081.
6
The E6/E7 promoter of extrachromosomal HPV16 DNA in cervical cancers escapes from cellular repression by mutation of target sequences for YY1.宫颈癌中染色体外HPV16 DNA的E6/E7启动子通过YY1靶序列突变逃避细胞抑制。
EMBO J. 1994 Mar 15;13(6):1460-6. doi: 10.1002/j.1460-2075.1994.tb06400.x.
7
TATA-binding protein-independent initiation: YY1, TFIIB, and RNA polymerase II direct basal transcription on supercoiled template DNA.不依赖TATA结合蛋白的起始：YY1、TFIIB和RNA聚合酶II在超螺旋模板DNA上指导基础转录。
Cell. 1994 Mar 25;76(6):1115-21. doi: 10.1016/0092-8674(94)90387-5.
8
Negatively cis-acting elements in the distal part of the promoter of Epstein-Barr virus trans-activator gene BZLF1.爱泼斯坦-巴尔病毒反式激活基因BZLF1启动子远端的负向顺式作用元件。
J Gen Virol. 1994 Aug;75 ( Pt 8):1999-2006. doi: 10.1099/0022-1317-75-8-1999.
9
The transcription factor YY1 binds to negative regulatory elements in the human cytomegalovirus major immediate early enhancer/promoter and mediates repression in non-permissive cells.转录因子YY1与人巨细胞病毒主要立即早期增强子/启动子中的负调控元件结合，并在非允许细胞中介导基因表达的抑制。
Nucleic Acids Res. 1994 Jul 11;22(13):2453-9. doi: 10.1093/nar/22.13.2453.
10
Interaction between transcription factors Sp1 and YY1.转录因子Sp1与YY1之间的相互作用。
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