成年仓鼠肝脏纯化生长抑制因子的小鼠对应物cDNA的分子克隆及序列分析

Molecular cloning and sequence analysis of the cDNA for the mouse counterpart to adult hamster liver purified growth inhibitory factor.

作者信息

Hashimoto C, Masuda H, Ayaki M, Suzuki Y, Uenaka A, Seya T, Miyoshi J, Takahashi K, Inui Y

机构信息

Center for Adult Diseases Osaka, Japan.

出版信息

Biochim Biophys Acta. 1997 Mar 1;1355(3):205-8. doi: 10.1016/s0167-4889(96)00180-2.

DOI:10.1016/s0167-4889(96)00180-2

PMID:9060991

Abstract

A cDNA clone encoding the mouse counterpart to adult hamster liver purified growth inhibitory factor (PGIF) was isolated from a mouse liver cDNA library by using antibodies raised against PGIF and sequenced. It contained a single open reading frame with a coding capacity for a 323 amino acid protein. Sequence analysis showed that it shared high homology with rat- and human liver arginases: the cDNA clone was 92% identical for rat arginase at the nucleotide level and was 93% identical to it at the deduced amino acid level. These results suggest that PGIF derived from adult hamster liver was identical or closely related to an isoform of hamster liver arginases.

摘要

通过使用针对成年仓鼠肝脏纯化生长抑制因子（PGIF）产生的抗体，从小鼠肝脏cDNA文库中分离出一个编码小鼠对应物的cDNA克隆，并进行了测序。它包含一个单一的开放阅读框，编码能力为一个323个氨基酸的蛋白质。序列分析表明，它与大鼠和人类肝脏精氨酸酶具有高度同源性：该cDNA克隆在核苷酸水平上与大鼠精氨酸酶的同源性为92%，在推导的氨基酸水平上与大鼠精氨酸酶的同源性为93%。这些结果表明，源自成年仓鼠肝脏的PGIF与仓鼠肝脏精氨酸酶的一种同工型相同或密切相关。