Aybay C, Imir T
Department of Microbiology & Immunology, Gazi University, Medical School, Ankara, Turkey.
Immunobiology. 1996;196(4):363-74. doi: 10.1016/S0171-2985(96)80059-3.
Candida albicans was studied for its capacity to induce TNF production from mouse peritoneal macrophages (PM phi). TNF activities in the culture supernatants of Candida-stimulated PM phi and human peripheral blood monocytes were assessed by L 929 bioassay and ELISA respectively. C. albicans induced TNF production from PM phi and human peripheral blood monocytes in a dose-dependent manner. Although the capacity was lesser than live form, heat-killed C. albicans was also found to be capable of stimulating PM phi to induce TNF. The filtered supernatant of 24 h cultured live C. albicans had no effects on TNF production from PM phi. Saccharomyces cerevisiae-extracted mannan, a yeast cell wall antigen, induced TNF production from PM phi in a dose-dependent manner. Thus, the effect of C. albicans on TNF production from PM phi was seemed to be directly related to the presence of the yeast cell wall itself. Compatible with these data, when various candida species (C. albicans, C. tropicalis, C. pseudotropicalis. C. lusitaniae, C. krusei, C. parapsilosis, C. guilliermondii, C. stellatoidea, C. glabrata) and S. cerevisiae were compared to each other, at a concentration of 2 x 10(6) yeast cells/ml from each species, it was observed that TNF inducing capacities varied. Among the species used in this study, C. guilliermondii and C. glabrata, of which the yeast cell size were the smallest ones, were found to be less potent than that of others to induce TNF from PM phi.
研究了白色念珠菌诱导小鼠腹腔巨噬细胞（PM phi）产生肿瘤坏死因子（TNF）的能力。分别通过L 929生物测定法和酶联免疫吸附测定法评估白色念珠菌刺激的PM phi和人外周血单核细胞培养上清液中的TNF活性。白色念珠菌以剂量依赖的方式诱导PM phi和人外周血单核细胞产生TNF。虽然其能力低于活菌体形式，但热灭活的白色念珠菌也被发现能够刺激PM phi诱导TNF。培养24小时的活白色念珠菌的过滤上清液对PM phi产生TNF没有影响。酵母细胞壁抗原酿酒酵母提取的甘露聚糖以剂量依赖的方式诱导PM phi产生TNF。因此，白色念珠菌对PM phi产生TNF的影响似乎与酵母细胞壁本身的存在直接相关。与这些数据一致，当将各种念珠菌属（白色念珠菌、热带念珠菌、伪热带念珠菌、葡萄牙念珠菌、克柔念珠菌、近平滑念珠菌、季也蒙念珠菌、星状念珠菌、光滑念珠菌）和酿酒酵母相互比较时，在每种菌浓度为2×10⁶个酵母细胞/毫升的情况下，观察到TNF诱导能力各不相同。在本研究使用的菌种中，酵母细胞尺寸最小的季也蒙念珠菌和光滑念珠菌被发现诱导PM phi产生TNF的能力比其他菌种弱。
