Di Massimo A M, Di Loreto M, Pacilli A, Raucci G, D'Alatri L, Mele A, Bolognesi A, Polito L, Stirpe F, De Santis R
Menarini Ricerche SpA, Department of Biotechnology, Pomezia, Rome, Italy.
Br J Cancer. 1997;75(6):822-8. doi: 10.1038/bjc.1997.147.
The present paper describes two immunoconjugates consisting of an anti-epidermal growth factor receptor (EGFR) monoclonal antibody (MAb), named Mint5, covalently linked to the type 1 ribosome-inactivating proteins (RIPs) ocymoidine (Ocy) and pyramidatine (Pyra) from Saponaria ocymoides and Vaccaria pyramidata respectively. Both antibody and toxins are shown to retain their respective biological properties upon chemical conjugation. The immunoconjugates exert specific inhibition of EGFR expressing target cell proliferation and protein synthesis in in vitro assays and also inhibit the growth of grafted human tumour cells in nude mice.
本文描述了两种免疫缀合物，它们由抗表皮生长因子受体（EGFR）单克隆抗体（MAb）组成，该单克隆抗体名为Mint5，分别与来自夏至草和王不留行的1型核糖体失活蛋白（RIPs）欧夏至草毒素（Ocy）和王不留行毒素（Pyra）共价连接。抗体和毒素在化学偶联后均显示保留其各自的生物学特性。在体外试验中，免疫缀合物对表达EGFR的靶细胞增殖和蛋白质合成具有特异性抑制作用，并且还能抑制裸鼠体内移植的人肿瘤细胞的生长。
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