Girardi G, Saball D E, Salvarrey M S, Elías M M
Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Consejo de Investigaciones de la Universidad Nacional de Rosario (CIUNR), Argentina.
J Biochem Toxicol. 1996;11(4):189-96. doi: 10.1002/(SICI)1522-7146(1996)11:4<189::AID-JBT4>3.0.CO;2-G.
We have examined the effects of mercuric chloride on renal glomerular structure. Isolated glomeruli from mercury-treated rats (HgCl2, 5 mg/kg body wt, s.c.) 1 hour post injection presented a diminished cross-sectional area as compared with control glomeruli [control (micron2) = 26,310 +/- 2,545, HgCl2 (micron2) = 18,474 +/- 1,828] and higher glomerular calcium content (control = 23 +/- 6 nmoles/mg prot, HgCl2 = 43 +/- 7 nmoles/mg prot). Renal sections prepared for immunohistochemical and histochemical analysis showed larger deposits of fibronectin and lipids and enhanced cellularity in glomerular structures from HgCl2-treated rats. Moreover, mieloperoxidase activity measured in isolated glomeruli were also increased as compared with control preparations [MPO (U/mg prot): control = 59 +/- 7, HgCl2 = 134 +/- 10]. When the animals were studied 24 hours post HgCl2 injection, glomerular cross-sectional area values were not different from control values (25,276 +/- 1,983 micron2), while calcium contents were higher than values observed 1 hour after treatment (92 +/- 9 nmoles/mg prot). A similar pattern was observed in fibronectin deposits. Hypercellularity in glomerular structures and the higher mieloperoxidase levels were maintained at this time (MPO HgCl2-rats 24 h = 148 +/- 31 U/mg prot). The effects observed in this study are consistent with an inflammatory response in the glomerular structure of HgCl2-treated rats that could explain the altered renal function described in previous reports in our laboratory.
我们研究了氯化汞对肾小球结构的影响。注射氯化汞（HgCl₂，5mg/kg体重，皮下注射）1小时后，从经汞处理的大鼠分离出的肾小球与对照肾小球相比，横截面积减小[对照（平方微米）=26,310±2,545，HgCl₂（平方微米）=18,474±1,828]，且肾小球钙含量更高（对照=23±6纳摩尔/毫克蛋白，HgCl₂=43±7纳摩尔/毫克蛋白）。为进行免疫组织化学和组织化学分析而制备的肾脏切片显示，HgCl₂处理的大鼠肾小球结构中纤连蛋白和脂质的沉积更大，细胞增多。此外，与对照制剂相比，分离出的肾小球中测得的髓过氧化物酶活性也有所增加[MPO（单位/毫克蛋白）：对照=59±7，HgCl₂=134±10]。在HgCl₂注射24小时后对动物进行研究时，肾小球横截面积值与对照值无差异（25,276±1,983平方微米），而钙含量高于处理后1小时观察到的值（92±9纳摩尔/毫克蛋白）。在纤连蛋白沉积中观察到类似模式。此时肾小球结构中的细胞增多和较高的髓过氧化物酶水平得以维持（HgCl₂处理大鼠24小时时MPO=148±31单位/毫克蛋白）。本研究中观察到的效应与HgCl₂处理的大鼠肾小球结构中的炎症反应一致，这可以解释我们实验室先前报告中描述的肾功能改变。
