Siddiqi Nikhat Jamal, Alhomida Abdullah Salih
Department of Biochemistry, King Saud University, College of Science, Riyadh, Saudi Arabia.
Med Sci Monit. 2006 Mar;12(3):BR95-101. Epub 2006 Feb 23.
The present study was undertaken to study the effect of mercuric chloride (HgCl2) on the collagen metabolism in rats and the protective effect of 2, 3-dimercapto-1-propane sulfonic acid (DMPS) in HgCl2-treated rats.
MATERIAL/METHODS: The experimental groups studied were (i) a control group (ii) rats injected with a single intraperitoneal (ip) dose of 2 mg of HgCl2/kg body weight (HgCl2-treated group, n=10 rats), (iii) Rats injected with a single dose (ip) of 100 mg of DMPS/kg body weight (DMPS group, n=10 rats), (iv) rats injected with a single dose (ip) of 100 mg of DMPS/kg body weight followed by a single dose (ip) of 2 mg of HgCl2/kg body weight 1 hour after a 100-mg DMPS injection (DMPS + HgCl2-treated group, n=10 rats). Half of the rats from each group were sacrificed after 24 hours and the other half after 48 hours of treatment.
A dose of 2.0 mg HgCl2/kg body weight caused an impairment of glomerular function, which was reflected by significant increases in the levels of serum creatinine and serum urea nitrogen in HgCl2-treated rats compared with control rats. Administration of 2.0 mg of HgCl2/kg body weight significantly increased urinary excretion of free hydroxyproline in HgCI2-treated rats compared with the control rats, reflecting increased collagen breakdown.
Administration of DMPS one hour before HgCl2 treatment caused the restoration of altered parameters to near normal levels.
本研究旨在探讨氯化汞（HgCl2）对大鼠胶原代谢的影响以及2,3-二巯基-1-丙烷磺酸（DMPS）对HgCl2处理大鼠的保护作用。
材料/方法：所研究的实验组包括：（i）对照组；（ii）腹腔注射（ip）单剂量2 mg HgCl2/kg体重的大鼠（HgCl2处理组，n = 10只大鼠）；（iii）腹腔注射单剂量100 mg DMPS/kg体重的大鼠（DMPS组，n = 10只大鼠）；（iv）腹腔注射单剂量100 mg DMPS/kg体重，1小时后再腹腔注射单剂量2 mg HgCl2/kg体重的大鼠（DMPS + HgCl2处理组，n = 10只大鼠）。每组一半的大鼠在处理24小时后处死，另一半在处理48小时后处死。
2.0 mg HgCl2/kg体重的剂量导致肾小球功能受损，这表现为与对照大鼠相比，HgCl2处理组大鼠血清肌酐和血清尿素氮水平显著升高。与对照大鼠相比，给予2.0 mg HgCl2/kg体重显著增加了HgCl2处理组大鼠尿中游离羟脯氨酸的排泄，反映出胶原分解增加。
在HgCl2处理前1小时给予DMPS可使改变的参数恢复到接近正常水平。
