Holmblad T, Thörnqvist P O, Söderhäll K, Johansson M W
Department of Physiological Botany, University of Uppsala, Sweden.
J Exp Zool. 1997 Feb 15;277(3):255-61.
We have cloned and sequenced a beta subunit of integrin from a cDNA library of crayfish hemocytes. This beta integrin shows great similarity to beta integrin subunits from other animals; the highest is towards beta pat-3 from Caenorhabditis elegans followed by beta PS from Drosophila melanogaster. By immunoblotting with antibodies raised towards a synthetic peptide corresponding to a part of the cytoplasmic region of the deduced protein sequence, it was shown that the integrin is present in the membrane of the hemocytes. This is the first integrin found in hemocytes of an invertebrate animal and this finding opens the door for further investigations on integrins and their role in the invertebrate immune system.
我们从小龙虾血细胞的cDNA文库中克隆并测序了一种整合素的β亚基。这种β整合素与其他动物的β整合素亚基具有高度相似性；与秀丽隐杆线虫的β pat - 3最为相似，其次是黑腹果蝇的β PS。通过用针对与推导的蛋白质序列的细胞质区域的一部分相对应的合成肽产生的抗体进行免疫印迹分析，表明该整合素存在于血细胞的膜中。这是在无脊椎动物血细胞中发现的首个整合素，这一发现为进一步研究整合素及其在无脊椎动物免疫系统中的作用打开了大门。
