Choi J S, Lee H J, Park K Y, Ha J O, Kang S S
Department of Food and Life Science, National Fisheries University of Pusan, Korea.
Planta Med. 1997 Feb;63(1):11-4. doi: 10.1055/s-2006-957593.
The antimutagenic activity of a methanol extract of Cassia tora seeds against aflatoxin B1(AFB1) was demonstrated with the Salmonella typhimurium assay. The numbers of revertants per plate decreased significantly when this extract was added to the assay system using Salmonella typhimurium TA100 and/or TA98. The MeOH extract was then sequentially partitioned with CH2Cl2, n-BuOH and H2O. The CH2Cl2 and n-BuOH fractions possessed antimutagenic activity but the H2O fraction was inactive. Neither the MeOH extract nor its fractions were capable of inhibiting the direct-acting mutagen N-methyl-N'-nitro-N-nitrosoguanidine suggesting that these fractions may prevent the metabolic activation of AFB1 or scavenge the electrophilic intermediate capable of inducing mutations. Column chromatography using silica gel yielded pure chrysophanol, chryso-obtusin, and aurantio-obtusin from the CH2Cl2 fraction and cassiaside and rubro-fusarin gentiobioside from the n-BuOH fraction. Each of these compounds demonstrated significant antimutagenic activity.
通过鼠伤寒沙门氏菌试验证明了决明子种子甲醇提取物对黄曲霉毒素B1（AFB1）的抗诱变活性。当将该提取物添加到使用鼠伤寒沙门氏菌TA100和/或TA98的试验系统中时，每平板回复突变体的数量显著减少。然后将甲醇提取物依次用二氯甲烷、正丁醇和水进行分配。二氯甲烷和正丁醇部分具有抗诱变活性，但水部分无活性。甲醇提取物及其部分均不能抑制直接作用的诱变剂N-甲基-N'-硝基-N-亚硝基胍，这表明这些部分可能会阻止AFB1的代谢活化或清除能够诱导突变的亲电中间体。使用硅胶柱色谱从二氯甲烷部分得到了纯的大黄酚、决明蒽醌和橙黄决明素，从正丁醇部分得到了决明子苷和红镰菌素龙胆二糖苷。这些化合物中的每一种都表现出显著的抗诱变活性。
