Antonov S, Zakhariev Z
Vet Med Nauki. 1977;14(3):61-7.
Studied were the activity and properties of alkaline phosphatase (AP) in the seminal plasma, spermatozoa washed with physiologic saline, testes, and accessory sexual glands of a bull. The AP activity was highest in the seminal plasma (2493.2 IU/l) and lowest in the Kupffer gland (257.3 IU/kg crude tissue, on an average). In washed spermatozoa it proved by 10 per cent lower than the activity in seminal plasma. At 56 degrees C as much as 60-80 per cent of the AP in the investigated materials was inactivated for 30 min. AP was shown to be inactivated strongly (up to 84-97 per cent) by urea (3.8 M). L-arginine (10(-2) M) and EDTA (10(-3) M) inactivated to an equal extent AP in all studied organs. L-phenylalanine inactivated AP weakly (13-15 per cent) in the testis and the epididymis, and more strongly (32-57 per cent) in the accessory glands and the plasma. Agar electrophoresis revealed three to four isoenzymes of AP in the seminal plasma, three isoenzymes in the testis and the epididymis, and two isoenzymes in the accessory sexual glands and in spermatozoa washed with physioogic saline.
研究了公牛精浆、用生理盐水洗涤的精子、睾丸和附属性腺中碱性磷酸酶（AP）的活性及特性。AP活性在精浆中最高（2493.2 IU/l），在库普弗腺中最低（平均每千克粗组织257.3 IU/kg）。在洗涤后的精子中，其活性比精浆中的活性低10%。在56摄氏度下，被研究材料中60%至80%的AP在30分钟内失活。结果表明，尿素（3.8 M）能使AP强烈失活（高达84%至97%）。L-精氨酸（10⁻² M）和乙二胺四乙酸（EDTA，10⁻³ M）在所有研究器官中使AP失活的程度相同。L-苯丙氨酸在睾丸和附睾中使AP微弱失活（13%至15%），而在附属性腺和精浆中使其失活更强（32%至57%）。琼脂糖电泳显示，精浆中有三到四种AP同工酶，睾丸和附睾中有三种同工酶，附属性腺和用生理盐水洗涤的精子中有两种同工酶。
