Zakhariev Z, Iotova M, Kichev G, Marinov M F, Al-Hanak H
Vet Med Nauki. 1983;20(2):27-32.
Studied was the effect of beta-glucuronidase, hydrocortisone, and mercaptoethanol in bull semen on the activity of the alkaline phosphatase and the viability and fertilizing capacity of spermatozoa. It was found that the beta-glucuronidase enzyme in conc. of 270 UI per cu. cm of semen enhanced the activity of one of the forms of AP in agar electrophoresis (AP-1) with the simultaneous enhancement of the thermal resistance of spermatozoa at 46 degrees C and their fertilizing capacity by 9.6 per cent at first insemination. At minimum concentration hydrocortisone (1 X 10(-6) M) lowered the heat resistance of spermatozoa at 39 degrees C. Mercaptoethanol was found to lower by 3 per cent the activity of semen alkaline phosphatase. It is suggested to use the beta-glucuronidase enzyme in the practice of artificial insemination out of all other biologically active agents studied.
研究了β-葡萄糖醛酸酶、氢化可的松和巯基乙醇对公牛精液中碱性磷酸酶活性以及精子活力和受精能力的影响。结果发现，精液浓度为每立方厘米270国际单位的β-葡萄糖醛酸酶可增强琼脂电泳中碱性磷酸酶一种形式（AP-1）的活性，同时提高精子在46摄氏度时的耐热性，且首次输精时其受精能力提高9.6%。氢化可的松最低浓度（1×10⁻⁶ M）会降低精子在39摄氏度时的耐热性。巯基乙醇可使精液碱性磷酸酶活性降低3%。在所有研究的生物活性剂中，建议在人工授精实践中使用β-葡萄糖醛酸酶。
