Ibrahim M, Suliman A, Hashim F A, Khalil el-T A, Evans D A, Kharazmi A, el Hassan A M
Institute of Endemic Diseases, University of Khartoum, Sudan.
Am J Trop Med Hyg. 1997 Jan;56(1):96-8. doi: 10.4269/ajtmh.1997.56.96.
A 45-year-old Sudanese man from western Sudan presented with oronasal leishmaniasis of three years duration. He had no history of previous kala-azar or cutaneous leishmaniasis. The parasite isolated from the oral mucosa was characterized by isoenzymes using 12 enzymes and by polymerase chain reaction amplification of kinetoplast DNA using species-specific primers. The specific primers gave products indistinguishable from those of the Leishmania donovani complex. However, the isoenzyme profile showed a zymodeme pattern that was significantly different from the zymodemes previously reported in the Sudan and the Ethiopian region.
