Lee K Y, Park J H, Chung M H, Park Y I, Kim K W, Lee Y J, Lee S K
College of Pharmacy, Seoul National University, Korea.
Biochem Mol Biol Int. 1997 Feb;41(2):285-92. doi: 10.1080/15216549700201291.
In the present study, we show that aloesin, which is a low molecular weight ingredients present in Aloe vera, stimulates the proliferation of cultured human hepatoma SK-HEP-1 cells. The incorporation of [3H] thymidine into DNA in the cell cultures was significantly increased at a dose of 10 microM aloesin. The aloesin-induced DNA synthesis appears to require newly synthesized proteins because cycloheximide treatment blocked the DNA synthesis evoked by this compound. We then examined whether this compound increases the intracellular levels of cell cycle regulators by immunoblotting. The data showed that aloesin increased the levels of cyclin E, CDK2, and CDC25A in SK-HEP-1 cells. In addition, immuno-complex kinase assays showed that aloesin up-regulated the enzyme activity of cyclin E/CDK2 kinase in a dose-dependent manner. Collectively, these results suggest that aloesin stimulates the proliferation of SK-HEP-1 cells by inducing the intracellular levels of cyclin E/CDK2 kinase complex and CDC25A, which, together, result in the up-regulation of cyclin E-dependent kinase activity.
在本研究中，我们发现芦荟素（一种存在于库拉索芦荟中的低分子量成分）可刺激培养的人肝癌SK-HEP-1细胞的增殖。在细胞培养物中，当芦荟素剂量为10微摩尔时，[3H]胸苷掺入DNA的量显著增加。芦荟素诱导的DNA合成似乎需要新合成的蛋白质，因为环己酰亚胺处理可阻断该化合物诱发的DNA合成。然后，我们通过免疫印迹法检测该化合物是否会增加细胞周期调节因子的细胞内水平。数据显示，芦荟素可增加SK-HEP-1细胞中细胞周期蛋白E、细胞周期蛋白依赖性激酶2（CDK2）和细胞分裂周期蛋白25A（CDC25A）的水平。此外，免疫复合物激酶分析表明，芦荟素以剂量依赖性方式上调细胞周期蛋白E/CDK2激酶的酶活性。总体而言，这些结果表明，芦荟素通过诱导细胞周期蛋白E/CDK2激酶复合物和CDC25A的细胞内水平来刺激SK-HEP-1细胞的增殖，这两者共同导致细胞周期蛋白E依赖性激酶活性的上调。
