Bando N, Tsuji H, Yamanishi R, Nio N, Ogawa T
Department of Nutrition, School of Medicine, The University of Tokushima, Japan.
Biosci Biotechnol Biochem. 1996 Feb;60(2):347-8. doi: 10.1271/bbb.60.347.
A major soybean allergen, Gly m Bd 30K, is a glycoprotein. A peptide containing a sugar chain was isolated from an alpha-chymotrypic hydrolyzate of the allergen. The amino acid sequence analysis of the peptide showed that its sugar chain binds to the Asn170 residue. Furthermore, the sugar moiety of the allergen and peptide was shown to consist of mannose, N-acetylglucosamine, fucose, and xylose at a molar ratio of 3 : 2 : 1 : 1. These results indicate that Gly m Bd 30 K is a N-linked glycoprotein.