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Identification of the glycosylation site of a major soybean allergen, Gly m Bd 30K.

作者信息

Bando N, Tsuji H, Yamanishi R, Nio N, Ogawa T

机构信息

Department of Nutrition, School of Medicine, The University of Tokushima, Japan.

出版信息

Biosci Biotechnol Biochem. 1996 Feb;60(2):347-8. doi: 10.1271/bbb.60.347.

DOI:10.1271/bbb.60.347
PMID:9063988
Abstract

A major soybean allergen, Gly m Bd 30K, is a glycoprotein. A peptide containing a sugar chain was isolated from an alpha-chymotrypic hydrolyzate of the allergen. The amino acid sequence analysis of the peptide showed that its sugar chain binds to the Asn170 residue. Furthermore, the sugar moiety of the allergen and peptide was shown to consist of mannose, N-acetylglucosamine, fucose, and xylose at a molar ratio of 3 : 2 : 1 : 1. These results indicate that Gly m Bd 30 K is a N-linked glycoprotein.

摘要

相似文献

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