Preparative electrophoresis of human lymphocytes. II. Use of non-immunoglobulin bearing lymphocytes obtained by electrophoretic levitation for the elicitation of a rabbit anti-human "T-cell" serum.

By intradermal injection (in Freund's complete adjuvant) of rabbits with only a few million human T-lymphocytes, obtained by preparative electrophoresis, an anti-human "T-cell" serum can be obtained. Although the antiserum is best used in a fairly concentrated form, its anti-human "T-cell" specificity (by immunofluorescence and E-rosetting) appears excellent, without the need to resort to absorption with various unwanted antigens. There may however, also be other specificities in the antiserum, as it also reacts with human granulocytes.