兔抗人胸腺细胞血清的制备及特异性检测（附1张彩色图片）

Preparation and specificity testing of a rabbit anti-human thymocyte serum (with 1 colour plate).

作者信息

Brutel de la Rivière A, Verhoef-Karssen P R, van Oers M H, Schoorl R, Feltkamp-Vroom T M, von dem Borne A E, Zeijlemaker W P

出版信息

Vox Sang. 1977;33(5):257-65. doi: 10.1111/j.1423-0410.1977.tb04471.x.

DOI:10.1111/j.1423-0410.1977.tb04471.x

PMID:72451

Abstract

The preparation of a specific anti-T cell serum, applicable in the indirect immunofluorescence technique on cell suspensions, smears of peripheral blood and bone marrow and on tissue sections is described. Rabbits were immunized with thymocytes; after removal of antibodies against species-specific antigens and antigens common to leucocytes by absorptions with red cells and granulocytes, specificity for thymocytes was obtained by repeated absorptions with CLL cells, used as B cell equivalents. Subsequently, the IgG fraction of the absorbed antiserum was isolated. This antibody preparation was tested with various types of blood cells as well as with cell suspensions depleted or enriched in T cells. For the study of tissue sections it had to be absorbed with liver powder. When studying lymphatic tissue it was found to stain only thymus-dependent areas in the specimens tested.

摘要

本文描述了一种特异性抗 T 细胞血清的制备方法，该血清适用于细胞悬液、外周血和骨髓涂片以及组织切片的间接免疫荧光技术。用胸腺细胞免疫兔子；通过用红细胞和粒细胞吸收去除针对种属特异性抗原和白细胞共有的抗原的抗体后，用慢性淋巴细胞白血病（CLL）细胞（用作 B 细胞等价物）反复吸收，获得了对胸腺细胞的特异性。随后，分离出吸收后抗血清的 IgG 部分。用各种类型的血细胞以及 T 细胞耗尽或富集的细胞悬液对该抗体制剂进行了测试。为了研究组织切片，必须用肝粉吸收。在研究淋巴组织时，发现它仅对测试标本中的胸腺依赖区染色。