Gonzalvo V, Navalón P, Lloris J M
Servicio de Investigaciones biotecnológicas, Hospital General Universitario de Valencia.
Actas Urol Esp. 1996 Oct;20(9):772-82.
The purpose of this paper is to present the changes that take place in testicular microcirculation measured by DLF during systemic administration of LHRH agonists. The essay includes a comparison with the variations registered in the volume of testicular interstitial fluid, the anatomopathological changes and the associated leucocyte demyeloperoxidase levels. We also examine the relationship between testicular microcirculation changes and plasma testosterone levels. To do this, 50 male Wistar rats were randomly divided into 5 groups, using 10 as control group and the remaining 40 distributed in 4 groups. Measurements were done at 2, 4, 8 and 24 hours after administration of Tryptorelin 0.4 mg i.v. We found that acute administration of an LHRH agonist causes a series of significant changes on testicular microcirculation. Testicular rhythmic microcirculatory flow, i.e., vasomotion, disappears. In turn, accumulation of PMN leucocytes associated to increased venular permeability takes places. Such pre- and postcapillary vascular changes lead to increased vascular permeability which results in increased volume of testicular interstitial fluid. This increased capillary permeability is responsible for the extensive interstitial oedema that would explain the serious histological changes seen on the seminiferous tubule with these drugs.
本文的目的是呈现静脉注射促黄体生成素释放激素（LHRH）激动剂时，通过激光散斑血流成像（DLF）测量的睾丸微循环变化。本文还包括与睾丸间质液体积变化、解剖病理学变化以及相关白细胞脱髓过氧化物酶水平的比较。我们还研究了睾丸微循环变化与血浆睾酮水平之间的关系。为此，将50只雄性Wistar大鼠随机分为5组，10只为对照组，其余40只分为4组。在静脉注射0.4mg曲普瑞林后2、4、8和24小时进行测量。我们发现急性注射LHRH激动剂会导致睾丸微循环发生一系列显著变化。睾丸有节律的微循环血流，即血管运动消失。反过来，与小静脉通透性增加相关的中性粒细胞会发生聚集。这种毛细血管前和毛细血管后的血管变化导致血管通透性增加，进而导致睾丸间质液体积增加。这种毛细血管通透性增加是导致广泛间质水肿的原因，这可以解释使用这些药物时在生精小管上看到的严重组织学变化。
