Zuo J, Rarey K E
Department of Otolaryngology, College of Medicine, University of Florida, Gainesville 32610, USA.
Eur Arch Otorhinolaryngol. 1997;254(2):73-7. doi: 10.1007/BF01526182.
The ultrastructural morphology of the interface region between the stria vascularis (SV) and spiral ligament (SL) was examined in the neonatal rat cochlea via transmission electron microscopy. At postnatal day (PND) 3, morphology of both basal cells and fibrocytes was simple and immature. Only a small number of fibrocytes was observed in the SL. Intercellular junctions between basal cells and fibrocytes, and between adjacent fibrocytes, were few. At PND 7, the number of fibrocytes increased, and more organelles appeared within their cytoplasm. From PND 11 to 14, nuclei of the basal cells appeared to be more spindle-shaped and contained more heterochromatin. The cytoplasm of the fibrocytes was pale, and a greater number of cytoplasmic vesicles and mitochondria emerged. More intercellular junctions were observed between basal cells and fibrocytes at the interface region and between fibrocytes in the SL. By PND 21, the morphology of basal cells and fibrocytes and their intercellular junctions appeared to be adult-like. These morphological observations correlate with previous reports on the functional maturation of the developing rat cochlea.
通过透射电子显微镜检查新生大鼠耳蜗中血管纹（SV）和螺旋韧带（SL）之间界面区域的超微结构形态。在出生后第3天（PND 3），基底细胞和成纤维细胞的形态都很简单且不成熟。在螺旋韧带中仅观察到少数成纤维细胞。基底细胞与成纤维细胞之间以及相邻成纤维细胞之间的细胞间连接很少。在PND 7时，成纤维细胞数量增加，其细胞质内出现更多细胞器。从PND 11到14，基底细胞的细胞核似乎更呈纺锤形，并且含有更多异染色质。成纤维细胞的细胞质呈浅色，出现了更多的细胞质小泡和线粒体。在界面区域的基底细胞与成纤维细胞之间以及螺旋韧带中的成纤维细胞之间观察到更多的细胞间连接。到PND 21时，基底细胞和成纤维细胞的形态及其细胞间连接似乎已接近成年状态。这些形态学观察结果与先前关于发育中大鼠耳蜗功能成熟的报道相关。
