新生大鼠耳蜗中钠钾ATP酶α和β亚型的表达

Expression of Na, K-ATPase alpha and beta isoforms in the neonatal rat cochlea.

作者信息

Zuo J, Curtis L M, Yao X, Ten Cate W J, Rarey K E

机构信息

Departments of Anatomy & Cell Biology and Otolaryngology, College of Medicine, University of Florida, Gainesville, USA.

出版信息

Acta Otolaryngol. 1995 Jul;115(4):497-503. doi: 10.3109/00016489509139355.

DOI:10.3109/00016489509139355

PMID:7572124

Abstract

The postnatal expression of five Na, K-ATPase alpha (alpha 1, alpha 2, alpha 3) and beta (beta 1, beta 2) subunit isoforms in the rat cochlea was investigated by immunocytochemistry. High levels of expression of the alpha 1 and beta 2 isoforms were observed in stria vascularis (SV) at all developmental stages. alpha 1 and beta 1 isoforms showed a distinct time-dependent developmental expression pattern in tissues of the spiral ligament (SL) and spiral limbus (SLi). Limited, temporary expression of alpha 2 and alpha 3 subunit isoforms were found in SV and SL. Expression of each isoform was also seen in organ of Corti (OC), spiral ganglion (SG), cochlear nerve (CN) and Kölliker's Organ (KO). These observations suggest that individual isoforms may exert specific actions postnatally during final cochlear maturation.

摘要

通过免疫细胞化学方法研究了大鼠耳蜗中五种钠钾ATP酶α（α1、α2、α3）和β（β1、β2）亚基异构体的产后表达情况。在所有发育阶段，血管纹（SV）中均观察到α1和β2异构体的高表达水平。α1和β1异构体在螺旋韧带（SL）和螺旋缘（SLi）组织中呈现出明显的时间依赖性发育表达模式。在SV和SL中发现α2和α3亚基异构体有有限的、暂时的表达。在柯蒂氏器（OC）、螺旋神经节（SG）、耳蜗神经（CN）和科蒂氏器（KO）中也可见到每种异构体的表达。这些观察结果表明，个体异构体可能在耳蜗最终成熟的产后阶段发挥特定作用。